[Bioperl-l] $hit_object->frac_aligned_hit/$hit_object->frac_aligned_query
Sendu Bala
bix at sendu.me.uk
Thu Aug 3 18:34:01 UTC 2006
Andreo Beck wrote:
[re: frac_aligned_query, frac_aligned_hit]
> It still gives more than 1. Can you tell me how good approximation it
> does? I mean if its 1.3 can I assume it 1 or something like that?
The only 'approximation' is to assume that none of the hsp alignments
have gaps. It is still impossible for it to get more than 1. When you
have gaps these methods are still useful because they give you a good
idea of how much of the hit/query was involved in the alignment - not
base for base but in terms of sequence coverage. So when they are
working without any bug, the number returned is valid and correct from a
certain viewpoint and shouldn't be 'fudged' like you suggest (treating
one number as another (1.3 != 1)).
If it still does get more than 1 it is a bug that needs to be fixed. For
the test data you sent me I get less than 1 now. Are you sure you
managed to get and install v.1.16 or higher of
Bio::Search::SearchUtils.pm ? Are you sure you are actually using the
new module when you run your script?
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