[Bioperl-l] In silico Chromosome Walking

[Ferdinand Marletaz]

Hi,

I'd like to find a script to make a kind of chromosome walking in  
matching pieces of cDNA on Traces of a Genome in order to find whole  
gene sequence from a little degenerate PCR fragment (100-500 pb).   
I've heard about  GenoTrace but it seems impossible to get it on the  
internet (link doesn't work http://rat.niob.knaw.nl/GENOTRACE/)  and  
I haven't understood if it requires full length cDNA or if it can  
work with shorter sequences (it would be possible to run GenScan on  
the previously obtained contig...)

Does anybody have the source of GenoTrace or an idea for my problem.

Thank you very much

Ferdi





___________________________________________
Ferdinand Marlétaz
Structure and Evolution of Nuclear Hormone Receptors
Laboratoire de Biologie Moléculaire de la Cellule (UMR 5161)
Ecole Normale Supérieure de Lyon
46, Allée d'Italie
69364 Lyon Cedex 07,  France
Tel. (33) 04 72 72 82 85
Port. 33 (0)6 30 35 58 49
Fax. (33) 04 72 72 80 80
Mail. Ferdinand.Marletaz at ens-lyon.fr