[Bioperl-l] retrieving sequences by ID
Sean O'Keeffe
limericksean at gmail.com
Wed Apr 27 10:33:14 EDT 2005
Hi all,
This is probably an easy problem for ye, but one I'm having difficulty
with none the
less.
I'm trying to extract only sequences from a fasta file (containing
~38,000 sequences)
containing a specific ID in the header line e.g.
return only the sequence for header containing 'ABCD12346' from:
>ABCD12345|followed by the rest of the description
acgtacgtgttttgggccctttaaa.....
>ABCD12346|description
acgtacgtgttttgggccctttaaa.....
>ABCD12347|description
acgtacgtgttttgggccctttaaa.....
...
The specific ID's are contained in a list(~20,000) which I want to loop through.
This is what I have done so far w/out any luck:
############################
use strict;
use lib "/usr/lib/perl5/site_perl/5.8.1/";
use Bio::SeqIO;
my (@ids)=@_
my $seq_in = Bio::SeqIO->new( '-format' => "fasta",
'-file' => "$fastafile");
my $seq_out = Bio::SeqIO->new( '-format' => "fasta",
'-file' => "$outfile");
for ($i=0;$i<=scalar(@ids);$i++){
while ($sequence = $seq_in->next_seq){
if ($sequence->display_id =~ /^>$ids[$i](.*$)/){
$seq_out->write_seq($sequence);
}
}
}
exit;
#############################
This has so far returned a list of 3 fasta headers and the programs
then finishes without errors.
I'd like to know where I'm going wrong and if possible, how I could
improve on things to prevent memory usage/speed it up.
Thanks in advance,
Sean O'Keeffe.
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