Not sure if this was fixed yet, but it is broken in my version #$Id: genbank2gff3.PLS,v 1.5 2005/01/17 19:48:57 cjm Exp $; Line 248 $fa_outfile =~ s/gff/fa/; if the string "gff" is in your --outfile, this creates problems It should be changed to $fa_outfile =~ s/gff$/fa/; -Michael Cipriano