[Bioperl-l] Blast Connection Error

Tue Apr 5 22:59:55 EDT 2005

Hello.

Until last two week, I could remote-blast against NCBI nr DB using bioperl
module. But right now, I can not remote-blast since a week.

I had set up interval time as 5 second. In my opinion, NCBI is not allowed
to blast search against NCBI nr DB. If you have experience like me, then
let me know.

Usually I do blast search for 50-100 sequences at one time.

The error message is as like this

-------------------- WARNING ---------------------

MSG: req was POST http://www.ncbi.nlm.nih.gov/blast/Blast.cgi

User-Agent: bioperl-Bio_Tools_Run_RemoteBlast/1.4

Content-Length: 336

Content-Type: application/x-www-form-urlencoded

DATABASE=ecoli&COMPOSITION_BASED_STATISTICS=off&QUERY=%3E1OSA%3A_+CALMODULIN
+-
+CHAIN+_+%0AAEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTID
FPEFLSLMARKMKEQDSEEELIEAFKVFDRDGNGLISAAELRHVMTNLGEKLTDDEVDEMIREADIDGDGHINYEE
FVRMMVSK&EXPECT=1e-
10&SERVICE=plain&FORMAT_OBJECT=Alignment&CMD=Put&FILTER=L&CDD_SEARCH=off&PRO
GRAM=blastp

<HTML>

<HEAD><TITLE>An Error Occurred</TITLE></HEAD>

<BODY>

<H1>An Error Occurred</H1>

500 Can't connect to www.ncbi.nlm.nih.gov:80 (Bad hostname
'www.ncbi.nlm.nih.gov')

</BODY>

</HTML>