[Bioperl-l] Problem with T-Coffee
Jonathan Manning
jon_r_manning at yahoo.com
Fri Aug 27 11:05:52 EDT 2004
Hi Jason,
Thanks for the reply.
My versions of Bioperl, Bioperl-run and T-Coffee are
the same on both machines.
When I run the test I get the following output:
1..22
# Running under perl version 5.008 for linux
# Current time local: Fri Aug 27 10:10:30 2004
# Current time GMT: Fri Aug 27 14:10:30 2004
# Using Test.pm version 1.23
ok 1
ok 2
ok 3
ok 4
ok 5
Abnormal Program Termination:[T-COFFEE, Version_1.37]
Please report the fault to:
cedric.notredame at europe.com (Indicate the version nu
mber)
Thank you for your cooperation :-)
------------- EXCEPTION -------------
MSG: TCoffee call crashed: 256 [command
/usr/bin/t_coffee -in=t/data/cysprot.fa,
Xblosum,Mlalign_id_pair,Mclustalw_pair -ktuple=3
-output=clustalw -outfile=/tmp
/MvE0LpuQko/mPn9bfUyI0 -quiet]
STACK Bio::Tools::Run::Alignment::TCoffee::_run
Bio/Tools/Run/Alignment/TCoffee.
pm:814
STACK Bio::Tools::Run::Alignment::TCoffee::align
Bio/Tools/Run/Alignment/TCoffee
.pm:719
STACK toplevel t/TCoffee.t:62
--------------------------------------
ok 6 # skip TCoffee program not found. Skipping.
ok 7 # skip TCoffee program not found. Skipping.
ok 8 # skip TCoffee program not found. Skipping.
ok 9 # skip TCoffee program not found. Skipping.
ok 10 # skip TCoffee program not found. Skipping.
ok 11 # skip TCoffee program not found. Skipping.
ok 12 # skip TCoffee program not found. Skipping.
ok 13 # skip TCoffee program not found. Skipping.
ok 14 # skip TCoffee program not found. Skipping.
ok 15 # skip TCoffee program not found. Skipping.
ok 16 # skip TCoffee program not found. Skipping.
ok 17 # skip TCoffee program not found. Skipping.
ok 18 # skip TCoffee program not found. Skipping.
ok 19 # skip TCoffee program not found. Skipping.
ok 20 # skip TCoffee program not found. Skipping.
ok 21 # skip TCoffee program not found. Skipping.
ok 22 # skip TCoffee program not found. Skipping.
I guess that explains something- it's not seeing the
T-Coffee program for some reason. I don't know why it
shouldn't- I placed it in /usr/bin using a soft-link
(ln -s).
Nothing unusual with my sequence file, but I'm
attaching it anyway, and relevant snippet of code is
below (it's a subroutine passed an arrayref of
sequences):
sub align {
# T-COFFEE USED TO CREATE A Bio::SimpleAlign OBJECT
my ($self) = shift;
my ( $relatives, $params ) = @_;
my @params = @{$params};
my $Aligner = new
Bio::Tools::Run::Alignment::TCoffee(@params);
my $aln = $Aligner->align($relatives);
return $aln;
}
I don't really have time to swap the program I'm
using, so I hope I can get this to work!
Thanks again- help appreciated.
Jon
--- Jason Stajich <jason at cgt.duhs.duke.edu> wrote:
> different versions of bioperl/bioperl-run on the dev
> machine and other
> machine? This happens for every every
> alignment you try on the non-dev machine? i.e.
> % perl -I. -w t/TCoffee.t
> in bioperl-run fails on the new machine too?
>
> An example sequence file and the script with at
> least the t-coffee part
> would help in debugging the problem if all of that
> is true.
>
> I've also moved to using MUSCLE instead of T-Coffee
> for some analyses
> because it is much faster and seems to be as
> accurate. The newest MAFFT
> is also rumoured to be as accurate too although one
> should always do their
> own tests on one's own data first...
>
> -jason
> On Fri, 27 Aug 2004, [iso-8859-1] Jonathan Manning
> wrote:
>
> > Hi to all,
> >
> > I've been using T-Coffee via the Bioperl wrapper
> as
> > part of a larger project. Though it worked fine on
> the
> > development system, it's not working on a
> different
> > one. It seems to do the pairwise stuff, at least
> it
> > gets to this stage:
> >
> > ......
> > AAH76853 CAG05593 : score= 400
> > AAH76853 CAE45989 : score= 410
> > CAG05593 CAE45989 : score= 460
> >
> >
> > MAKE NEIGHBOR JOINING DENDROGRAM
> > [MODE=fast] [Output dendrogram
> file=5XIMxfCi1F.dnd]
> >
> >
> > But then it crashes out with the following
> messages:
> >
> > Abnormal Program Termination:[T-COFFEE,
> Version_1.37]
> > Please report the fault to:
> > cedric.notredame at europe.com (Indicate the version
> > number)
> > Thank you for your cooperation :-)
> >
> > ------------- EXCEPTION -------------
> > MSG: TCoffee call crashed: 256 [command
> > /usr/bin/t_coffee
> >
>
-in=/tmp/5XIMxfCi1F,XBLOSUM,Mlalign_id_pair,Mclustalw_pair
> > -ktuple=2 -output=clustalw
> > -outfile=/tmp/6wIuLkvcGl/w7jE5bLbih]
> >
> > STACK Bio::Tools::Run::Alignment::TCoffee::_run
> >
>
/usr/lib/perl5/site_perl/5.8.0/Bio/Tools/Run/Alignment/TCoffee.pm:814
> > STACK Bio::Tools::Run::Alignment::TCoffee::align
> >
>
/usr/lib/perl5/site_perl/5.8.0/Bio/Tools/Run/Alignment/TCoffee.pm:719
> > STACK SSIR::align
> > /usr/lib/perl5/site_perl/5.8.0/SSIR.pm:2094
> > STACK toplevel /var/www/cgi-bin/ssir.cgi:557
> >
> > --------------------------------------
> >
> > Similar commands work fine from the command line.
> >
> > Does anyone have any ideas?
> >
> > Many thanks,
> >
> > Jon
> >
> >
> >
> >
> >
> >
> >
>
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> >
>
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> >
>
> --
> Jason Stajich
> Duke University
> jason at cgt.mc.duke.edu
>
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-------------- next part --------------
>XP_058513 PREDICTED: similar to RIKEN cDNA 4921513O20; cDNA sequence, clone 3-75 [Homo sapiens].
MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNI
HVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILK
MLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLG
CKALHVLFERGNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNI
LVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEED
KLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSM
LMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKM
LNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTE
FHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGA
MDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQ
TKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMD
DYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSG
SREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDK
TSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDS
VFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDL
LKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCC
ISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDV
SRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSP
LRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKF
SCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWS
RVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLN
FDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQ
SATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQ
AEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDY
HFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEF
PVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCK
IMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEE
YLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRER
ALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHID
SLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQ
PRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAV
KIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKAS
LTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCC
RMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDE
LEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRIL
LPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALV
HLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLV
GTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFS
FSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCG
LIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLI
RVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQ
NLEKHIEVRKELAEKMRRTSVE
>CAD39185 hypothetical protein [Homo sapiens].
VFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTE
GYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTC
LYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSEST
NSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAK
QNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWI
GTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQK
EIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE
>BAB29628 unnamed protein product [Mus musculus].
MLGAIFERPIPPDTRPWYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCR
MGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDEL
AIQGKLPDPVKEYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILI
PKNIIVECMVATNLNSKSATLWLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVH
LAAEKESWVVCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVG
TADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSF
SNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCEL
IDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIR
TIHNFCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQN
LEKHIEVRTELADKMRKTSVE
>AAH34074 4921513O20Rik protein [Mus musculus].
SEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAY
CLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETL
LKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRN
IMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHL
HHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHS
AMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGN
VIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVKEYGCAPWPMV
EKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMVATNLNSKSATL
WLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWVVCGTQSGALLVI
NVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMIFEDKAVKCKGAAP
LKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSFSNDFTIQKLIETKTNQLFSYA
AFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCELIDCVHFLKEVMVKLNKESKHQ
LSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNFCDSVRAMATAQLGSLK
NVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKMRKTSVE
>XP_235581 similar to RIKEN cDNA 4921513O20; cDNA sequence, clone 3-75 [Rattus norvegicus].
MASGACQGCDEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDILVFTYSDRASKLFEGK
NVHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLI
LKMLTVHHANVNLSIVGLKALDLLLDSGKITLLILDEECDVFLLIFDAMHRYSANEEVQK
LACKALHVLFERVSEEQLTEFVENKDYMTLLSTFRSFKRDEEIVHHVLCCLHSLAVTCSN
VEVLMSGNVRCYNIVVEAMKTFPTSENIQEVSCSLLHKLTLGNFFNILVLNEVHVFVVKA
VQRYPENVALQISALSCLALLNLEERSETQENSDEDSEKPFWLEPCYKALMRHRKNKHVQ
EAACWALNNLLMYQSSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLE
QNGLPLASIINGGMACICTHKVSLVHAPMSSILVLPSSMKILLAKGVYLNVLELMQRHAQ
VPEVAESGCKMLSHLFEGSNPSLDTVAAVIPKILTVMRTHGTSLSVQLEALRALLHFVVP
GVSEDSRDDSRCQPNVLRTQCFRTDIHKLVLAALNRFIGNPGIQKCGLKVISSFAHLPDA
LEMLSLHGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKILASTLQ
RFKDVAEVQTTGLQTVLSMLDLSVSFSKLLVHYSFDVVMFHQMSSGVLEQKDEQFLNLCC
KCFAKVAVDDELKSKMLERACDQNNSIMVECLLLLGADANQAKGATSLIYQVCEKESSPK
LVELLLNSGCREQDVRKALTVSIQKGDNQVISLLLRRLALDLANNSICLGGFCIGKLDPS
WLGPLFPDKSSNLRKQTNAGSVLARKVLRYQMRNTLQEGVASGSEGNFSEDALAKFGEWT
FIPDSSMDSVFGQSDDLDSEGSESSFLVKKKSNSVSVGEVYRDLALQRCSPNAQRHSSSL
GPVFDHEDLLRRKRKILSSDESLRSSRLQSHTRQSDSSSSLASEREHITSLDLSANELKD
IDALGQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLTHLDLHSNKFATFPSFMLK
MPSVIHLDASRNDIGPTVVLDPVVKCPSLKQFNLSYNQLSSIPENLDQVVEKLEQLLLEG
NKISGICSPLSLKELKILNLSKNHIPSLPEDFLEACPKVESFSARMNFLAVHTVSLPPTG
FILVCFVKIPPEIGRLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHI
GCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKMKKSELGMQGATVGI
DVRDWPIQIRGKRKKDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAM
KPWLFNIKARASSSPVILVGTHLDVSDEKQRKACIGKITKELLNKRGFPTIRDYHFVNAT
EESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRK
HLLQLVKEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQIL
TVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMAQYFKLLEKFQIALPIGEEYLLVPS
SLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNR
MYWRQGIYLNWSPEAYCLVGSEVLDSRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEW
FPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIP
ISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKH
TSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQ
HRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKT
SEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGNRIMEGLRFPNEFDELAIQGK
LPDPVKEYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHIFIPKDIT
VECIAATNLNSKRATLWLGCGNTEKGQLSLLDLNTERYSYEEVTDSRILCLALVHLAAEK
ESWVVCGTQSGALLVINAEDETRRHTLDKMTDSVTCLYCNSFAKQSKQSHFLLVGTADGN
LMIFEDKTIKCKGAAPLKTLHIGDVSTPLMCLSESMNSSERHITWGGCGTKIFSFSNDFT
IQKLIETRTNQLFSYSAFSDSNIIAVAVDTALYIAKKNSPVVENYKSCDAGDRSQATCVQ
NSEMTTALPPSCFKVLAGCTRLGRSVKSLSADRTVNEHILYASREVMVKINKDSKHKLSY
SGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNFCDSKLSMFCFGKGSLKNVM
LVLGYKRKSTEGTQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKMRKTSVE
>AAH35949 4921513O20Rik protein [Mus musculus].
CLALVHLAAEKESWVVCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQS
NFLLVGTADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCG
TKVFSFSNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKT
EKLCELIDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLS
TRRVIRTIHNFCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNL
PHEVQNLEKHIEVRTELADKMRKTSVE
>XP_415940 PREDICTED: similar to hypothetical protein [Gallus gallus].
MKVYEILNSAELICLMRYVSIPSTSTAECMVAANQSCKKAGVWIGNGNTEKAQLSFVNLN
TDGHTCEDIADSKILSLALVTLPTEKENWIVAGTHSGSLWAVNTEDETRRHRLQKMSDSV
TCLYCNSLSKQSKQKNFFLVGTADGTLAVFEDRAVKCKGATPLKILTIGNVSTPLMCLNE
STYLSEKNTIWGGCGTKLIALSSDFSVQKLIETKSSQLFCHNAYSDANIISIAVDKSIYL
AKKNSHFVEIWDKKTEKLSELIDCAHFLKDEVEKLNKESKHKVAYSGRVKTICLQKNTAL
WVGTGGGHILLLDLSTRRPVRVIDNFCDSIRVMLTAQLGSLKNVVLVLGYCYKSDTEDDK
SHKELQSCVSVWDINLPHEVQNLKKHIEHSYNTATARIALWPRTGPGSPCPVTGRSDRAH
SSSPAHTAMAESHRAAHREPCYVSAGPAAFLYLGISAVTWKASVCPRHPQLLQHSKLKRY
TTGAKASAQNNVGWAPFSSQSRADVSPSHPDQTSQEGAFGGEPEAHPLPAALLGQQAALF
PPHLNAVAAALCQQLVAVQSMLSNCIARFLSNCAGYLELSSTQQPLKSLQRSPQGIGEWG
GAPSPGHSQTHHSSSQHDCKLPPEHSAKAGTAAVGP
>AAH76853 Unknown (protein for IMAGE:6637919) [Xenopus laevis].
IAPDIVLADLPRNIMLNAEELEFDQAPEFLLGDGGFGSVYRASYKGEDVAVKIFNKHTSS
RLLRQELTVLSHLHHPSLVYLLAAGVHPRMLVMELAPKGSLDHLLQQDSASLTRTLQHRI
ALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNSAIIAKIADYGIAQYCCRMGIKTSEG
TPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTGGARMVEGLKFPNEFDELAIHGRLPD
PVKEYNCAPWPEVEVLIKKCLKENPQQRPTSVTVYEILNSAELLCLMRNHVIFSMLTAEC
MVAASPLVKKHVVWVGNGCTEKGQMCCIDLNKGGQTCEDFSDSRILCLTLVTLPGEKQNW
IVAGTQSGEIITADLDELKIKHTLHKLTDSVTCLLFSCIQGQSQKKYHILAGTANGRIAI
FENTAVKCAYAAPIKVIDIGDNSTPVMCLCESTYSSEKNSVWGICGTKIFSLSSEFTVQK
TIDTRTATLYSHGSYSESNITCFAIDKYIYVAKKISPYVEIWDKKTEKICEVLDCVQFLK
EDSRKRRKCKQEEAYTSRVKALYLQKNTALWVGTGGGHILLIDLSTRQPIRIITSFCDSI
RSMVPAQLDRGSIKNVVLVLGCRCTPQKEIQSFLSVWDTNLPHEVQNLKKHNEIRQEFAD
KLRGLSMD
>CAG05593 unnamed protein product [Tetraodon nigroviridis].
MVNGEELEEQLRKLLVRLKSQQQERQLGTLIQILQDLLFLAHTDEAAELFEDKDVHVSLT
VALSSYASRRGVQQVGWSLLCRLMEICPGTLERLTAAQPAAEDSQVLPVHKLVLKVLSQH
GADCKVTMVGLRALALLLRSDAVPPLLLEDESEDVLGLVVQAMKVFPVNEEVLLHGCGAL
QVLLETVSEDHLVEFVENQDHVVVLAALQTFLDNPELLLRGLKVLLPLARPGGVRCYSTV
IAAMDAFPEVEDLQETACRLFRAFTLESYYNILVRNGVQRVAACCTALDLHAEEPDVQEA
ASWAIERLLVHGAGPRPAEEDQEERTPVHRQLMAALLLHPKAPSVFQAATSAISTLIQQD
GERSTPRAAVKRCLVPPTFAAFAGRTRAPLLSSGLHVNLVEMMKRHWTSAEVSISACRLL
SLLFQGRPASLDELNMTMSQILCTIKAHNFNPEVQLEALRASLVLLYPDRSLREHGVSVA
DPDMADVSLKVLKNQCVLEGAHTLYLEVLNRFISSSAIQKSGLKVLSALADCSGAVDLLC
QQGAIDTVLHTLQMFPGEREIHYWGFTLLNYLVTKKKLSPMIVPVLASVLVASLVQYRED
SEMTLKCFQVALRMLDASPRSAAELQRENFDRQIFQQLREDTADLCNYPLRKSVCLALFK
MWSDSELRYAMLEKACQDGDATMAECLIELGADVNAKTKSESLLYQVGGENLCVHRSVLT
AGPSVPQVCERGGPLELAELLVRRGAHEQHLRRALAVSVKRADGPAVILLLARLGLDHNS
SALCLGGFRLGRLDAGWLGPLLAERRSARSLCRTSSKAVSLARFVQSFQRSKSHSSVLRS
PVDPCLTSGYISEESDDSNFSFVSVDDGQIPNEDMESDGSDSLPGVLSPEDPQALRRRQG
RQRHPSPEVQPPHQRPGCVGVFFSLFAPVFVFLFQNGQTETEPCDSVQKRYAKSPQKVFR
PADGPAALQKVQDRVRLLDLSANELDALSCLMDDGVVQQQLGHLLRLDLSHNSLQEFPSS
LCQSLSSLTRLDLQGNQLPSLPVELLTLQSLNTLNISRNCVGPQLTFDPAVSCPSLRHLN
LSFNKIAAFPHQLSGAGSQLEELCLEGNCIRELCVPLSLPEIKLLDVSKNSVETVSADFL
TGCPKLETLNVSVNKISSLSHLPSKLTTLKLANNGFAEVSAAILGLPNLRSVDMRTNRIA
ALPGPASWESCNLRELNFSQNRIGTLDLSGPVHRWARLEKLHLSDNQLSEIPPQIGLLEG
LNSLDVSRNANLHSFPDEMGKLGRLWDLPLDGLRLQLDLKLIGNKTKDIVRFLQQRLKKA
VPYHRMKLIVVGNVASGKTTLIQQLMKLKRSQLKSKSTSVGIDVQDWTIKDWDRKKMVLN
VWDFSGGEEFSGSHPHFLTSRALYLVVYNLSRGASQVDALKPWLFNIKAAAPVSPVILVG
THTDVSNELQLQACLTKIREELLSHQSFPAIRDYHMVSASEDSDALTKLRKAIAREVANF
KIQGQPVMGQLVPDSYVELERQLLQERARVPPEFPVLRHREVLQLIQENQLQLEEAELPH
AIHFLSEAGQNFHELFFPLLTVLSDFLDFRSGNVELRSCSEEGRLYSHLLVFPAGVLLHF
DDPALQLQNLYFIDPQWLCHIISQKLTLKSCGFWEPPKGVIQRSLVEKFLSETKCFPKSH
MTQLFKLLEKFQIALPFGEDQLLVPSRWAHGRSVQRQHTAVCVFVSVGNPTDVGSVFGPS
LSKHRPVIELPHGENSEVIVRLYEMPYFPMDFWSRQIIRLLEFSSHLLSGREKAVRPNRI
YWRRGVYLSWSPDAYCLVEAASVEDNPSSFVRITVPSSQKGRVLLGQVVDHVDSLLEEWF
PGLLSTNMHGSGEALLKKWALYSFEDGQEWSKVLLDDLCPHLDAGVLLQDEEQPGLALPL
SQVAPDLVLSDQPAGLMLDSAELSVDLSERLGTPATRPEPRLGVLLSDNVLFPGGGGFGN
VYRGVYRKEDVAVKIFNNHASELYVFRLLRQELVVLSRLRHPSLVALLAASTAARALVME
LAPRGSLDALFQHENGGLNRKLQHRIALQVADGLRYLHSAMIVYRDLKPHNVLLFNLKTD
CEMIAKITDYGIAQHCCSMGVRSCEGTPGGLSSWAWFCSRFLPVQRQFVLGVQALGFRLC
KAPGGERRVVDGCASAGFRAPEVARGNVAYNQQADVFSFGLLLYDLLTCGERISDGMKFP
SEFDEIAVQRKLPDPVKHYGCAPWPGLQALMRDCLKESPQCRPTSAQVFDRLNSGEVLCL
MRELAVPRVPSAECLALSGGCTAWLGGGSSAQRRGSVTAVNLHTNAVTTEAIDTSPVLCL
TTVQIPNQTSDWLVAGTQSGALVVLSSQDALTWHRLQSVRDAVTSLFFHLHPCRNQRKNY
LLVGTADGTVTIYEDSVLKQEDGRPVKTVTVGQLDTPVVCLGQSLHWQDSRCVWAGCGTK
VLSFTADYDLGRSVDTRPSAEFQQEQSWASEACVSRMVVDKHVYLSKAGSPLVEVWDKKS
ERMVDRIDCAHIIRRDSGGTTEGSSSWARVKALLVQSAAALWIGTRGGYLLLLELSKHQP
LQVIGPRCDSVRCMAPALIETPNWKNVVLVLGRRLPQGRNQADEESVLMVWNGMLPMEAK
DLKRHCHRREQVAVRMR
>CAE45989 hypothetical protein [Homo sapiens].
TEKLCGLIDCVHFLREVTVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDL
STRRLIRVIYNFCNSVRVMMTAQLGKFLSFRYFSYSLSLIKYAFILRLHFL
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