[Bioperl-l] Bio:Tools::Primer3, Bio::Seq::PrimedSeq, and Bio::SeqFeature::Primer

[Rob Edwards]

I am glad that the modules help.

The problem is weird because $tempfile and $temphandle are created by 
Root::IO, so this shouldn't be the only module that fails. Do you have 
File::Temp installed on your system? (try perldoc File::Temp). What system 
are you running on?

It seems that Root::IO is returning the filename but not the filehandle, and 
I am not sure why. 

Perhaps others can weigh in with suggestions?

Rob

On Friday 04 April 2003 10:13 am, anthony.underwood at hpa.org.uk wrote:
> Dear Rob,
>
> Thankyou very much for putting together the primer3 modules for bioperl.
> They really are extremely useful. However I've run into a problem with the
> Run/Primer3.pm module
>
> At line 341
>  print $temphandle join "\n", @{$self->{'primer3_input'}}, "=\n";
>
> This doesn't produce any data in the temporary file and none of the test
> scripts work due to primer3 failing  However if I replace it with the text
> below then all tests work.
>
>
>  open (INPUT, ">$tempfile");
>  print INPUT join "\n", @{$self->{'primer3_input'}}, "=\n";
>  close INPUT;
>
>
> Is this is a setup problem with my machine?
>
> Thanks for our help,
>
>
> Anthony