[Biojava-l] [BioSQL-l] Load/Update Genbank sequence issue
gang wu
gwu at molbio.mgh.harvard.edu
Wed May 21 06:28:16 UTC 2008
I got another problem with almost the same code(see attached code and
output). If I call the loadSeq() only once, everything works fine. The
second call of the same method will fail. By the exception message, it
looks like the session is not cleaned up yet though at the end of the
method the session has been flush/clear/closed. Anything wrong?
Or does anyone have a sequence data loader would like to share? I'm
quite frustrated after keeping trying to do this seems simple thing
without success. If I can make it work, I'd like to post it on the
mailing list. Thanks in advance.
Gang
====================================================
public class SequenceLoaderNew {
public void loadSeq(String fileName, String namespace) throws
FileNotFoundException, BioException, SQLException, InterruptedException {
SessionFactory sessionFactory = new
Configuration().configure().buildSessionFactory();
Session session = sessionFactory.openSession();
RichObjectFactory.connectToBioSQL(session);
Namespace ns = (Namespace)
RichObjectFactory.getObject(SimpleNamespace.class, new Object[]{namespace});
RichSequenceDB db = new BioSQLRichSequenceDB(session);
System.out.println("Start loading file " + fileName + " at " +
System.currentTimeMillis());
BufferedReader br = new BufferedReader(new FileReader(fileName));
RichSequenceIterator rsi =
RichSequence.IOTools.readGenbankDNA(br, ns);
try {
while (rsi.hasNext()) {
RichSequence sequence = rsi.nextRichSequence();
System.out.println("Loaded sequence" +
sequence.getAccession() + ", identifier: " + sequence.getIdentifier());
Transaction tx = session.beginTransaction();
try {
System.out.println("Delete sequence" +
sequence.getAccession() + " if exists.");
db.removeSequence(sequence.getAccession());
tx.commit();
session.flush();
session.clear();
} catch (Exception ex) {
if (tx != null) {
tx.rollback();
}
}
try {
tx = session.beginTransaction();
System.out.println("Save sequence " +
sequence.getAccession());
//session.save("BioEntry", sequence);
//db.addSequence(sequence);
db.addRichSequence(sequence);
tx.commit();
System.out.println("Finished savig sequence " +
sequence.getAccession() + "\n");
} catch (HibernateException ex) {
tx.rollback();
ex.printStackTrace();
}
}
} finally {
session.flush();
session.clear();
session.close();
}
}
public static void main(String[] args) throws BioException,
FileNotFoundException, InterruptedException, SQLException {
SequenceLoaderNew sl = new SequenceLoaderNew();
sl.loadSeq("/genomeseq/bacteria/NC_005813.gbk", "genbank");
sl.loadSeq("/genomeseq/bacteria/NC_005813.gbk", "genbank");
}
}
====================================================
Start loading file /genomeseq/bacteria/NC_005813.gbk at 1211349859602
Loaded sequenceNC_005813, identifier: 45478502
Delete sequenceNC_005813 if exists.
Save sequence NC_005813
Finished savig sequence NC_005813
Start loading file /genomeseq/bacteria/NC_005813.gbk at 1211349863761
Loaded sequenceNC_005813, identifier: 45478502
Delete sequenceNC_005813 if exists.
Save sequence NC_005813
org.hibernate.NonUniqueObjectException: a different object with the same
identifier value was already associated with the session: [Term#20]
at
org.hibernate.engine.StatefulPersistenceContext.checkUniqueness(StatefulPersistenceContext.java:590)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.performUpdate(DefaultSaveOrUpdateEventListener.java:284)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsDetached(DefaultSaveOrUpdateEventListener.java:223)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.performSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:89)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:70)
at
org.hibernate.impl.SessionImpl.fireSaveOrUpdate(SessionImpl.java:507)
at org.hibernate.impl.SessionImpl.saveOrUpdate(SessionImpl.java:499)
at
org.hibernate.engine.CascadingAction$5.cascade(CascadingAction.java:218)
at org.hibernate.engine.Cascade.cascadeToOne(Cascade.java:268)
at org.hibernate.engine.Cascade.cascadeAssociation(Cascade.java:216)
at org.hibernate.engine.Cascade.cascadeProperty(Cascade.java:169)
at org.hibernate.engine.Cascade.cascade(Cascade.java:130)
at
org.hibernate.event.def.AbstractSaveEventListener.cascadeBeforeSave(AbstractSaveEventListener.java:431)
at
org.hibernate.event.def.AbstractSaveEventListener.performSaveOrReplicate(AbstractSaveEventListener.java:265)
at
org.hibernate.event.def.AbstractSaveEventListener.performSave(AbstractSaveEventListener.java:181)
at
org.hibernate.event.def.AbstractSaveEventListener.saveWithGeneratedId(AbstractSaveEventListener.java:107)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.saveWithGeneratedOrRequestedId(DefaultSaveOrUpdateEventListener.java:187)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsTransient(DefaultSaveOrUpdateEventListener.java:172)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.performSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:94)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:70)
at
org.hibernate.impl.SessionImpl.fireSaveOrUpdate(SessionImpl.java:507)
at org.hibernate.impl.SessionImpl.saveOrUpdate(SessionImpl.java:499)
at
org.hibernate.engine.CascadingAction$5.cascade(CascadingAction.java:218)
at org.hibernate.engine.Cascade.cascadeToOne(Cascade.java:268)
at org.hibernate.engine.Cascade.cascadeAssociation(Cascade.java:216)
at org.hibernate.engine.Cascade.cascadeProperty(Cascade.java:169)
at
org.hibernate.engine.Cascade.cascadeCollectionElements(Cascade.java:296)
at org.hibernate.engine.Cascade.cascadeCollection(Cascade.java:242)
at org.hibernate.engine.Cascade.cascadeAssociation(Cascade.java:219)
at org.hibernate.engine.Cascade.cascadeProperty(Cascade.java:169)
at org.hibernate.engine.Cascade.cascade(Cascade.java:130)
at
org.hibernate.event.def.AbstractSaveEventListener.cascadeAfterSave(AbstractSaveEventListener.java:456)
at
org.hibernate.event.def.AbstractSaveEventListener.performSaveOrReplicate(AbstractSaveEventListener.java:334)
at
org.hibernate.event.def.AbstractSaveEventListener.performSave(AbstractSaveEventListener.java:181)
at
org.hibernate.event.def.AbstractSaveEventListener.saveWithGeneratedId(AbstractSaveEventListener.java:107)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.saveWithGeneratedOrRequestedId(DefaultSaveOrUpdateEventListener.java:187)
at
org.hibernate.event.def.DefaultSaveEventListener.saveWithGeneratedOrRequestedId(DefaultSaveEventListener.java:33)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsTransient(DefaultSaveOrUpdateEventListener.java:172)
at
org.hibernate.event.def.DefaultSaveEventListener.performSaveOrUpdate(DefaultSaveEventListener.java:27)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:70)
at org.hibernate.impl.SessionImpl.fireSave(SessionImpl.java:535)
at org.hibernate.impl.SessionImpl.save(SessionImpl.java:523)
at primerdb.SequenceLoaderNew.loadSeq(SequenceLoaderNew.java:51)
at primerdb.SequenceLoaderNew.main(SequenceLoaderNew.java:69)
Exception in thread "main" org.hibernate.NonUniqueObjectException: a
different object with the same identifier value was already associated
with the session: [Term#19]
at
org.hibernate.engine.StatefulPersistenceContext.checkUniqueness(StatefulPersistenceContext.java:590)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.performUpdate(DefaultSaveOrUpdateEventListener.java:284)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsDetached(DefaultSaveOrUpdateEventListener.java:223)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.performSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:89)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:70)
at
org.hibernate.impl.SessionImpl.fireSaveOrUpdate(SessionImpl.java:507)
at org.hibernate.impl.SessionImpl.saveOrUpdate(SessionImpl.java:499)
at
org.hibernate.engine.CascadingAction$5.cascade(CascadingAction.java:218)
at org.hibernate.engine.Cascade.cascadeToOne(Cascade.java:268)
at org.hibernate.engine.Cascade.cascadeAssociation(Cascade.java:216)
at org.hibernate.engine.Cascade.cascadeProperty(Cascade.java:169)
at org.hibernate.engine.Cascade.cascade(Cascade.java:130)
at
org.hibernate.event.def.AbstractSaveEventListener.cascadeBeforeSave(AbstractSaveEventListener.java:431)
at
org.hibernate.event.def.AbstractSaveEventListener.performSaveOrReplicate(AbstractSaveEventListener.java:265)
at
org.hibernate.event.def.AbstractSaveEventListener.performSave(AbstractSaveEventListener.java:181)
at
org.hibernate.event.def.AbstractSaveEventListener.saveWithGeneratedId(AbstractSaveEventListener.java:107)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.saveWithGeneratedOrRequestedId(DefaultSaveOrUpdateEventListener.java:187)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsTransient(DefaultSaveOrUpdateEventListener.java:172)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.performSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:94)
at
org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:70)
at
org.hibernate.impl.SessionImpl.fireSaveOrUpdate(SessionImpl.java:507)
at org.hibernate.impl.SessionImpl.saveOrUpdate(SessionImpl.java:499)
at
org.hibernate.engine.CascadingAction$5.cascade(CascadingAction.java:218)
at org.hibernate.engine.Cascade.cascadeToOne(Cascade.java:268)
at org.hibernate.engine.Cascade.cascadeAssociation(Cascade.java:216)
at org.hibernate.engine.Cascade.cascadeProperty(Cascade.java:169)
at
org.hibernate.engine.Cascade.cascadeCollectionElements(Cascade.java:296)
at org.hibernate.engine.Cascade.cascadeCollection(Cascade.java:242)
at org.hibernate.engine.Cascade.cascadeAssociation(Cascade.java:219)
at org.hibernate.engine.Cascade.cascadeProperty(Cascade.java:169)
at org.hibernate.engine.Cascade.cascade(Cascade.java:130)
at
org.hibernate.event.def.AbstractFlushingEventListener.cascadeOnFlush(AbstractFlushingEventListener.java:131)
at
org.hibernate.event.def.AbstractFlushingEventListener.prepareEntityFlushes(AbstractFlushingEventListener.java:122)
at
org.hibernate.event.def.AbstractFlushingEventListener.flushEverythingToExecutions(AbstractFlushingEventListener.java:65)
at
org.hibernate.event.def.DefaultFlushEventListener.onFlush(DefaultFlushEventListener.java:26)
at org.hibernate.impl.SessionImpl.flush(SessionImpl.java:1000)
at primerdb.SequenceLoaderNew.loadSeq(SequenceLoaderNew.java:60)
at primerdb.SequenceLoaderNew.main(SequenceLoaderNew.java:69)
====================================================
Richard Holland wrote:
> Forgot to copy my reply back to the lists!
>
>
> ---------- Forwarded message ----------
> From: Richard Holland <dicknetherlands at gmail.com>
> Date: 2008/5/20
> Subject: Re: [BioSQL-l] Load/Update Genbank sequence issue
> To: gang wu <gwu at molbio.mgh.harvard.edu>
>
>
> You need to substitute this line:
>
> RichSequenceIterator rsi = RichSequence.IOTools.readGenbankDNA(br, new
> SimpleNamespace(namespace));
>
> with this line:
>
> Namespace ns = (Namespace)RichObjectFactory.getObject(SimpleNamespace.class,new
> Object[]{namespace});
> RichSequenceIterator rsi = RichSequence.IOTools.readGenbankDNA(br, ns);
>
> cheers,
> Richard
>
> PS. You're the second person who has quoted this section of code on
> this list recently, and had the same problem with it. If you got it
> from a website somewhere, could you send me the URL so I can get in
> touch with the authors and get them to correct it? Thanks!
>
> 2008/5/20 gang wu <gwu at molbio.mgh.harvard.edu>:
>
>> Hi all,
>>
>> I'm trying to load Genbank sequence into BioSQL database with
>> BioJavax/Hibernate. It's Ok to load the first sequence. Then I got the
>> exception when loading new sequences or reloading the same sequence. Seems
>> the Hibernate is trying to insert "genbank" namespace again. Can anybody
>> tell me what I did wrong? Thanks in advance.
>>
>> Gang
>>
>> ================================================================
>> public class SequenceLoaderNew {
>> public void loadSeq(String fileName, String namespace) throws
>> FileNotFoundException, BioException {
>> System.out.println("Start loading file " + fileName + " at " +
>> System.currentTimeMillis());
>> BufferedReader br = new BufferedReader(new FileReader(fileName));
>> Session session = new
>> Configuration().configure().buildSessionFactory().openSession();
>> RichObjectFactory.connectToBioSQL(session);
>> RichSequenceDB db = new BioSQLRichSequenceDB(session);
>> RichSequenceIterator rsi = RichSequence.IOTools.readGenbankDNA(br, new
>> SimpleNamespace(namespace));
>> while (rsi.hasNext()) {
>> Transaction tx = session.beginTransaction();
>> RichSequence sequence = rsi.nextRichSequence();
>> System.out.println("Loaded sequence" + sequence.getAccession() +
>> ", identifier: " + sequence.getIdentifier());
>> try {
>> System.out.println("Delete sequence" + sequence.getAccession()
>> + " if exists.");
>> db.removeSequence(sequence.getAccession());
>> } catch (Exception ex) {
>> }
>> try {
>> System.out.println("Save sequence " +
>> sequence.getAccession());
>> session.save("BioEntry", sequence);
>> tx.commit();
>> session.flush();
>> session.clear();
>> } catch (HibernateException ex) {
>> tx.rollback();
>> ex.printStackTrace();
>> }
>> }
>> }
>>
>> public static void main(String[] args) throws BioException,
>> FileNotFoundException {
>> SequenceLoaderNew sl = new SequenceLoaderNew();
>> //sl.loadSeq("/genomeseq/bacteria/NC_005813.gbk", "genbank");
>> sl.loadSeq("/genomeseq/bacteria/NC_004349.gbk", "genbank");
>> }
>> }
>> ================================================================
>> org.hibernate.exception.ConstraintViolationException: could not insert:
>> [Namespace]
>> at
>> org.hibernate.exception.SQLStateConverter.convert(SQLStateConverter.java:71)
>> at
>> org.hibernate.exception.JDBCExceptionHelper.convert(JDBCExceptionHelper.java:43)
>> at
>> org.hibernate.id.insert.AbstractReturningDelegate.performInsert(AbstractReturningDelegate.java:40)
>> at
>> org.hibernate.persister.entity.AbstractEntityPersister.insert(AbstractEntityPersister.java:2163)
>> at
>> org.hibernate.persister.entity.AbstractEntityPersister.insert(AbstractEntityPersister.java:2643)
>> at
>> org.hibernate.action.EntityIdentityInsertAction.execute(EntityIdentityInsertAction.java:51)
>> at org.hibernate.engine.ActionQueue.execute(ActionQueue.java:279)
>> at
>> org.hibernate.event.def.AbstractSaveEventListener.performSaveOrReplicate(AbstractSaveEventListener.java:298)
>> at
>> org.hibernate.event.def.AbstractSaveEventListener.performSave(AbstractSaveEventListener.java:181)
>> at
>> org.hibernate.event.def.AbstractSaveEventListener.saveWithGeneratedId(AbstractSaveEventListener.java:107)
>> at
>> org.hibernate.event.def.DefaultSaveOrUpdateEventListener.saveWithGeneratedOrRequestedId(DefaultSaveOrUpdateEventListener.java:187)
>> at
>> org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsTransient(DefaultSaveOrUpdateEventListener.java:172)
>> at
>> org.hibernate.event.def.DefaultSaveOrUpdateEventListener.performSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:94)
>> at
>> org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:70)
>> at
>> org.hibernate.impl.SessionImpl.fireSaveOrUpdate(SessionImpl.java:507)
>> at org.hibernate.impl.SessionImpl.saveOrUpdate(SessionImpl.java:499)
>> at
>> org.hibernate.engine.CascadingAction$5.cascade(CascadingAction.java:218)
>> at org.hibernate.engine.Cascade.cascadeToOne(Cascade.java:268)
>> at org.hibernate.engine.Cascade.cascadeAssociation(Cascade.java:216)
>> at org.hibernate.engine.Cascade.cascadeProperty(Cascade.java:169)
>> at org.hibernate.engine.Cascade.cascade(Cascade.java:130)
>> at
>> org.hibernate.event.def.AbstractSaveEventListener.cascadeBeforeSave(AbstractSaveEventListener.java:431)
>> at
>> org.hibernate.event.def.AbstractSaveEventListener.performSaveOrReplicate(AbstractSaveEventListener.java:265)
>> at
>> org.hibernate.event.def.AbstractSaveEventListener.performSave(AbstractSaveEventListener.java:181)
>> at
>> org.hibernate.event.def.AbstractSaveEventListener.saveWithGeneratedId(AbstractSaveEventListener.java:107)
>> at
>> org.hibernate.event.def.DefaultSaveOrUpdateEventListener.saveWithGeneratedOrRequestedId(DefaultSaveOrUpdateEventListener.java:187)
>> at
>> org.hibernate.event.def.DefaultSaveEventListener.saveWithGeneratedOrRequestedId(DefaultSaveEventListener.java:33)
>> at
>> org.hibernate.event.def.DefaultSaveOrUpdateEventListener.entityIsTransient(DefaultSaveOrUpdateEventListener.java:172)
>> at
>> org.hibernate.event.def.DefaultSaveEventListener.performSaveOrUpdate(DefaultSaveEventListener.java:27)
>> at
>> org.hibernate.event.def.DefaultSaveOrUpdateEventListener.onSaveOrUpdate(DefaultSaveOrUpdateEventListener.java:70)
>> at org.hibernate.impl.SessionImpl.fireSave(SessionImpl.java:535)
>> at org.hibernate.impl.SessionImpl.save(SessionImpl.java:523)
>> at primerdb.SequenceLoaderNew.loadSeq(SequenceLoaderNew.java:38)
>> at primerdb.SequenceLoaderNew.main(SequenceLoaderNew.java:52)
>> Caused by:
>> com.mysql.jdbc.exceptions.MySQLIntegrityConstraintViolationException:
>> Duplicate entry 'genbank' for key 'name'
>> at com.mysql.jdbc.SQLError.createSQLException(SQLError.java:1011)
>> at com.mysql.jdbc.SQLError.createSQLException(SQLError.java:956)
>> at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java:3491)
>> at com.mysql.jdbc.MysqlIO.checkErrorPacket(MysqlIO.java:3423)
>> at com.mysql.jdbc.MysqlIO.sendCommand(MysqlIO.java:1936)
>> at com.mysql.jdbc.MysqlIO.sqlQueryDirect(MysqlIO.java:2060)
>> at com.mysql.jdbc.ConnectionImpl.execSQL(ConnectionImpl.java:2542)
>> at
>> com.mysql.jdbc.PreparedStatement.executeInternal(PreparedStatement.java:1734)
>> at
>> com.mysql.jdbc.PreparedStatement.executeUpdate(PreparedStatement.java:2019)
>> at
>> com.mysql.jdbc.PreparedStatement.executeUpdate(PreparedStatement.java:1937)
>> at
>> com.mysql.jdbc.PreparedStatement.executeUpdate(PreparedStatement.java:1922)
>> at
>> org.hibernate.id.IdentityGenerator$GetGeneratedKeysDelegate.executeAndExtract(IdentityGenerator.java:73)
>> at
>> org.hibernate.id.insert.AbstractReturningDelegate.performInsert(AbstractReturningDelegate.java:33)
>> ... 31 more
>> ================================================================
>>
>> _______________________________________________
>> BioSQL-l mailing list
>> BioSQL-l at lists.open-bio.org
>> http://lists.open-bio.org/mailman/listinfo/biosql-l
>>
>>
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