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Hi,</div>
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i am new here so I am sorry if I am doing anything wrong, feel free to tell me.</div>
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I am farely new to biopython as well, currently i have written a script that reads in a .gbk file and then deletes and inserts different parts of the sequence. this would alter the absolute position of features. I have ultimately run a script that safes the
new positions in 2 dataframes. When i now want to update all features with the Featurelocation command it seems to not work. I saved the features in a variable before and after me updating the features and was hoping to then detect a difference but it fails.
here the basic part of the script i am struggling with</div>
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CompareBefore = Seqfile.features</div>
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for z in range(0, len(featureEndList)):
<div> feature_end = int(ELdf.iloc[z][1])</div>
<div> feature_start = int(SLdf.iloc[z][1])</div>
<div> feature_strand = Seqfile.features[z].location.strand</div>
<div> Seqfile.features[z].location = FeatureLocation(feature_start, feature_end,strand=feature_strand)</div>
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<span style="background-color:rgb(255, 255, 255);display:inline !important">CompareAfter = Seqfile.features</span><br>
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list = []
<div>for x in range(0,len(CompareAfter)):</div>
<div> if CompareBefore[x] != CompareAfter[x]:</div>
list.append(x)<br>
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I get 0 hits in this list.</div>
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Please help <span id="🙂">🙂 thank you</span></div>
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