<div dir="ltr"><div>I misread. I was not aware that Biopython provided an interface to call primer3. That's neat. I guess a tutorial could be useful.</div><div><br></div><div><br></div><div>primer3-py wraps around the primer3 library and lets you want to compute Tm, deltaG and other primer-specific information. It's quite useful if you are doing more than just running primer3.<br></div></div><div id="DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2"><br>
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</table><a href="#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2" width="1" height="1"></a></div><div class="gmail_extra"><br><div class="gmail_quote">On Thu, May 11, 2017 at 6:02 PM, Jocelyne <span dir="ltr"><<a href="mailto:jocelyne@gmail.com" target="_blank">jocelyne@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">I would recommend this library: <a href="https://pypi.python.org/pypi/primer3-py" target="_blank">https://pypi.python.<wbr>org/pypi/primer3-py</a><div><br></div></div><div class="gmail_extra"><br><div class="gmail_quote"><div><div class="h5">On Thu, May 11, 2017 at 5:36 PM, Shyam Saladi <span dir="ltr"><<a href="mailto:saladi@caltech.edu" target="_blank">saladi@caltech.edu</a>></span> wrote:<br></div></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div><div class="h5"><div dir="ltr">Hi --<div><br></div><div>Is there a tutorial for designing primers with Primer3 through Biopython? I didn't see anything in the cookbook (other than as it relates to <a href="http://biopython.org/DIST/docs/tutorial/Tutorial.html#htoc287" target="_blank">sequence matching</a>), and a cursory search seemed to only turn up this <a href="http://biopython.org/DIST/docs/presentations/biopython.pdf" target="_blank">presentation</a>.</div><div><br></div><div>I could probably put a tutorial together based on the code I've written for a project just now if this seems like a useful thing to others ...</div><div><br></div><div>Thanks,</div><div>Shyam</div></div>
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