<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><h1 class="topic-post-title" id="yui_3_14_1_1_1430758283783_2049" style="color: rgb(68, 68, 68); margin: 0px 0px 15px; line-height: 24px; padding-right: 30px;"><span style="font-size: 12px; font-weight: normal;"><u>Does anyone know of a python script for codon optimization of a FASTA file using a codon usage table as a reference?</u></span></h1><div class="topic-post-text" id="yui_3_14_1_1_1430758283783_2042" style="line-height: 21px; margin-bottom: 10px; color: rgb(68, 68, 68);"><p id="yui_3_14_1_1_1430758283783_2041" style="margin: 0px 0px 10px; padding-right: 0px;">Hi, I need a template Python script (or a complete script or perhaps just some advice) for conversion of a gene belonging to <em>R. eutrophia</em> for heterologous expression in <em>C. acetobutylicum. </em>I have a codon usage table as a .txt file copied from Kazusa and the gene I'm interested in as FASTA. If anyone has a .py file they can upload that would be great :)</p><div>Kind regards,</div><div>Ryan H.</div><div><br></div><div><a href="https://www.researchgate.net/post/Does_anyone_know_of_a_python_script_for_codon_optimization_of_a_FASTA_file_using_a_codon_usage_table_as_a_reference">(previously posted on research gate)</a></div></div></body></html>