<div dir="ltr"><div class="gmail_default" style="font-size:small">Hi,</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small">The pydna package ( <a href="https://pypi.python.org/pypi/pydna/">https://pypi.python.org/pypi/pydna/</a> )</div><div class="gmail_default" style="font-size:small">has pcr functionality.</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small">have a look at the documentation</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style><a href="http://pydna.readthedocs.org/en/latest/pydna.html#module-pydna.amplify">http://pydna.readthedocs.org/en/latest/pydna.html#module-pydna.amplify</a><br></div><div class="gmail_default" style><br></div><div class="gmail_default" style>cheers,</div><div class="gmail_default" style>Bjorn</div></div><div class="gmail_extra"><br><div class="gmail_quote">On Sun, Mar 29, 2015 at 1:00 PM, <span dir="ltr"><<a href="mailto:biopython-request@mailman.open-bio.org" target="_blank">biopython-request@mailman.open-bio.org</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Send Biopython mailing list submissions to<br>
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Today's Topics:<br>
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1. Determine thermocycler sequence for PCR (Horea Christian)<br>
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Message: 1<br>
Date: Sat, 28 Mar 2015 23:20:52 +0100<br>
From: Horea Christian <<a href="mailto:h.chr@mail.ru">h.chr@mail.ru</a>><br>
To: <a href="mailto:biopython@mailman.open-bio.org">biopython@mailman.open-bio.org</a><br>
Subject: [Biopython] Determine thermocycler sequence for PCR<br>
Message-ID: <<a href="mailto:1427581252.22280.12.camel@mail.ru">1427581252.22280.12.camel@mail.ru</a>><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Hi, do you guys know any good literature reference that provides a<br>
reliable equation to determine the ideal step temperatures and durations<br>
for a PCR given the primers and the amplicon?<br>
<br>
I see annealing temps can be calculated with biopython via<br>
SeqUtils.MeltingTemp, which is nice, but it would be even greater if we<br>
could provide assistance with designing more of the PCR protocol. I am<br>
asking this also because I have a set of new primers over here which<br>
likely need a new thermocycler protocol, and all I found via google was<br>
very vague.<br>
<br>
BEst,<br>
Christian<br>
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End of Biopython Digest, Vol 147, Issue 13<br>
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</blockquote></div><br><br clear="all"><div><br></div>-- <br><div class="gmail_signature"><div dir="ltr"><font face="garamond, serif">______O_________oO________oO______o_______oO__<br>Björn Johansson<br>Assistant Professor<br>Departament of Biology<br>University of Minho<br>Campus de Gualtar<br>4710-057 Braga<br>PORTUGAL<br><a href="http://www.bio.uminho.pt" target="_blank">www.bio.uminho.pt</a><br><a href="https://profiles.google.com/bjornjobb" target="_blank">Google profile</a></font><div><font face="garamond, serif"><a href="http://scholar.google.com/citations?user=7AiEuJ4AAAAJ" target="_blank">Google Scholar Profile</a><br>my <a href="https://sites.google.com/site/metabolicengineeringgroup/" target="_blank">group</a><br>Office (direct) +351-253 601517 | (PT) mob. +351-967 147 704 | (SWE) mob. +46 739 792 968<br>Dept of Biology (secr) +351-253 60 4310 | fax +351-253 678980</font></div></div></div>
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