[Bioperl-l] Grail Tool

Alan R Williams alanrw at cs.manchester.ac.uk
Mon May 16 10:43:40 EDT 2005


Hello,

I am trying to use Bio::Tools::Grail to parse a Grail output file.  It 
reads the file OK but does not produce any predictions.  Is there a 
particular part of the Grail output that it understands?  I've attached 
one of the test Grail outputs I'm using.

Thanks in advance,

Alan
-------------- next part --------------
gc_object_start: gene_grailexp --organism human --output pretty --dbpat grailexp_v3 

# Service: gene_grailexp
# Version: 3.3
# Description: GAT GrailEXP Gene Prediction Service
# Last Modified: October, 2001
# Tool:  GrailEXP 3.3 from ORNL.  Last updated:  October, 2001.
# Database:  GrailEXP Database Thu Feb 27 16:15:37 EST 2003 from NCBI/TIGR/Baylor/Riken (15960696 entries).  Last updated:  Thu Feb 27 16:15:37 2003.
# Sequence Name: >gene_grailexp|PID=9167
# Sequence Length: 18553
# Output_begin: pretty
--------------------------------------------------------------------------------
GrailEXP v3.31 [March, 2002]                   http://compbio.ornl.gov/grailexp/

Authors:  Doug Hyatt, Manesh Shah, Victor Olman, Richard Mural, Ying Xu, and 
  Edward C. Uberbacher, 1996-2001

Reference:  "Automated Gene Identification in Large-Scale Genomic Sequences",
  Xu, Y. and Uberbacher, E.C., Journal of Computational Biology, Volume 4,
  Number 3, 1997

Sequence:  >gene_grailexp|PID=9167 (18553 bp)
--------------------------------------------------------------------------------
GAWAIN Gene Predictions (1 predicted, 1 with database similarity)



Genes with Database Similarity (1 predicted, 0 with alternative splices)


Gene 1, Variant 1        Strand: +  Bounds: 1-393  Exons: 1
                         Start Codon:  No     Stop Codon:  Yes

Top-Scoring Reference:  BF958402.1 (542 bp) (89% id, 1-393)
     >human|BF958402.1|est_human|dbEST - gi|12375677|gb|BF958402.1|BF958402
      QV4-NN1148-291100-616-h10 NN1148 Homo sapiens cDNA
Reference Path:  BF958402.1 (542 bp) (89%, 1-393)

 ---Index---- --------Exons-------- ---------CDS--------- -Ph- -Fr- -Len- -Scr-
 1.1.1                 1        393          1        259   2    1    393   90


>GrailEXP Gene 1, Var 1 mRNA|Similar to BF958402.1
tgaattcagtgttaccactgcatatccagcccatctgacaccttcagatatgaagctgctcccatcagta
aagtattcgacatctgggtcttttaagggctggtcctttatgtcttttcggcttgagaaaacctcatcca
gtatttccacacaacagtcatacccagggccacacaacatgggctttccatgctctacccattctattgg
cagcagtgtggctggatttagagtattcacaatctccaaggttatgtaggggttttcacacaaaagccct
tgatatcttaacatcctagggttagaaagccagcgatgcccactctgctccatcagggtgatgaccgtgt
ggggaacccgaattatcaacctttgtcccaatttgagcttgtt

>GrailEXP Gene 1, Var 1 protein|Derived from similarity to BF958402.1
EFSVTTAYPAHLTPSDMKLLPSVKYSTSGSFKGWSFMSFRLEKTSSSISTQQSYPGPHNMGFPCSTHSIG
SSVAGFRVFTISKVM*


Genes with No Database Evidence (0 predicted)


--------------------------------------------------------------------------------
GALAHAD Gene Alignments (4 located: 3 displayed, 1 redundant)

 Index Std    Begin       End         Accession     Database   Organism   Length

      1 +          1        393         BF958402.1    est_human      human   542

  1 piece    Seq exons = (1..393)
 89% ident   Ref exons = (148..542)

      2 +        402        676         BF906163.1    est_human      human   354

  1 piece    Seq exons = (402..676)
 88% ident   Ref exons = (4..277)

      3 +        402        676         BI020262.1    est_human      human   355

  1 piece    Seq exons = (402..676)
 88% ident   Ref exons = (5..278)

--------------------------------------------------------------------------------
PERCEVAL Exon Candidates (6 predicted)

 Index Std   Begin       End     Frm    Type     Len    Scr    Quality

      1 +        337        481   2   Terminal    145    63    Marginal
      2 -       1362       1482   2   Internal    121    19        Poor
      3 +       6947       7042   0    Initial     96    41    Marginal
      4 -       7856       7943   0    Initial     88    16        Poor
      5 -      14834      15109   0   Internal    276    69    Marginal
      6 -      15364      15448   0    Initial     85    59    Marginal
--------------------------------------------------------------------------------
# Output_end: pretty

gc_object_end: gene_grailexp --organism human --output pretty --dbpat grailexp_v3 

gc_object_start: cpg_grailexp --output pretty

# Service: cpg_grailexp
# Version: 3.0
# Description: GAT GrailEXP CpG Island Locator
# Last Modified: December 4, 2000
# Tool:  GrailEXP 3.3 from ORNL.  Last updated:  October, 2001.
# Sequence Name: >cpg_grailexp|PID=9476
# Sequence Length: 18553
# Output_begin: pretty
--------------------------------------------------------------------------------
GrailEXP v3.31 [March, 2002]                   http://compbio.ornl.gov/grailexp/

Authors:  Doug Hyatt, Manesh Shah, Victor Olman, Richard Mural, Ying Xu, and 
  Edward C. Uberbacher, 1996-2001

Reference:  "Automated Gene Identification in Large-Scale Genomic Sequences",
  Xu, Y. and Uberbacher, E.C., Journal of Computational Biology, Volume 4,
  Number 3, 1997

Sequence:  >cpg_grailexp|PID=9476 (18553 bp)
--------------------------------------------------------------------------------
PERCEVAL CpG Islands (2 predicted)

 Index    Begin       End        Ratio    Pct_GC

      1       5791       6610     0.90     67.32
      2       7044       7412     0.85     58.88
--------------------------------------------------------------------------------
# Output_end: pretty

gc_object_end: cpg_grailexp --output pretty

gc_object_start: repeat_grailexp --output pretty

# Service: repeat_grailexp
# Version: 2.0
# Description: GAT GrailEXP Repetitive Element Locator
# Last Modified: December 4, 2000
# Tool:  GrailEXP 3.3 from ORNL.  Last updated:  October, 2001.
# Database:  Repeatmasker Repetitive Database Apr 20 from GIRI (4760 entries).  Last updated:  Fri Feb 28 13:12:30 2003.
# Sequence Name: >repeat_grailexp|PID=9508
# Sequence Length: 18553
# Output_begin: pretty
--------------------------------------------------------------------------------
GrailEXP v3.31 [March, 2002]                   http://compbio.ornl.gov/grailexp/

Authors:  Doug Hyatt, Manesh Shah, Victor Olman, Richard Mural, Ying Xu, and 
  Edward C. Uberbacher, 1996-2001

Reference:  "Automated Gene Identification in Large-Scale Genomic Sequences",
  Xu, Y. and Uberbacher, E.C., Journal of Computational Biology, Volume 4,
  Number 3, 1997

Sequence:  >repeat_grailexp|PID=9508 (18553 bp)
--------------------------------------------------------------------------------
PERCEVAL Repeats (11 located:  2 simple, 9 complex)


Simple Repeats (2 located)

 Index    Begin       End          Score    1st 10 Bases

      1      12069      12175         76    ttttctgttt...
      2      14222      14323         58    atggagataa...

Complex Repeats (9 located)

 Index Std   Begin       End         E-Val             Element Names

      1 +      16656      16793     2e-218    FAM#SINE/Alu/FAM#SINE/Alu_/7SL...
      2 -       8079       8186     6e-154    L1PA14#LINE/L1/L1PA13#LINE/L1/...
      3 -       3319       3360      0.006        HERV15#LTR/ERV1___LTR15_5%...
      4 -       3081       3147      7e-18    HERV3#LTR/ERV1___15%_div,_with...
      5 -       2559       2927      5e-32        HERV15#LTR/ERV1___LTR15_5%...
      6 -       1791       1867      4e-07        HERV15#LTR/ERV1___LTR15_5%...
      7 -       1154       1254      2e-05    HERV3#LTR/ERV1___15%_div,_with...
      8 -        581        849      4e-29    HERV3#LTR/ERV1___15%_div,_with...
      9 -         49         90      2e-05    HERV3#LTR/ERV1___15%_div,_with...

Masked Sequence

>repeat_grailexp|PID=9508, masked
tgaattcagtgttaccactgcatatccagcccatctgacaccttcagannnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttttaagggctggtcctttatgtcttttcg
gcttgagaaaacctcatccagtatttccacacaacagtcatacccagggccacacaacat
gggctttccatgctctacccattctattggcagcagtgtggctggatttagagtattcac
aatctccaaggttatgtaggggttttcacacaaaagcccttgatatcttaacatcctagg
gttagaaagccagcgatgcccactctgctccatcagggtgatgaccgtgtggggaacccg
aattatcaacctttgtcccaatttgagcttgttagcatcttctgctaacaggacagtggc
tgccaatgccttgaaacacagtggccatcccatagccaccaagtctagccctttggacta
atatgccatgggccaataccatgaccctagtatttgtaccaagactcctgtagctattcc
ttaactttcatgaacatataggtaaaaaggctttttcatgnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnntacagaagcctaaatactggacacttttaaagcagatttgagcctttttta
cctgacactttatatcctgccttccacagcaggtgcagaaggttttgggtttttcccggg
tgcagtagcccttggccagtggctttcttataccatggtcctgaccattttccttattct
cttgacattcatcctttcagtgtcctttccttttgcaccattcacattaatctttttcta
gccttggccagctcttaaatccctgtccagactggcctctttcatgactgcacccacgcc
tccttgcaaagtcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgtagc
cactttagtaagctgagtatcactcacgcctgtggagcttctaacttctggaatttatgc
ctgatatttccctgggcctgccttacaaatgccatatttaccatgtactgtttttcagca
gcctcagggtcaaatggcatatgaagccgataagcctcgcagagcctctcgtaaaattca
ccgggactttcatctggcttctggtggacctctgagacctttctgatattggtggccttt
ttccctccagcctttattccatttaggagtgcctctcaataccactgcaaactttgtggc
ccttgagcctggttagggtcccagcctgggacagcctctatcagaagtgcttgttgtgca
tactgcctgatatctcctgtgcctgcaggctcattgttctcgagccactggagagcagct
tgtataactctttggtgctcttccatatcaaataatgacagaagaagttgtttgcaatca
acccaggtaggattgtgcattaggaaaatgggctgcattaaatctataagnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnntgaagagccattctcccccttggacttcattctgtgcattcaaataaatttgt
ccccatgtttatcggagggacatctgcattgcttgggcatgacctgacctaagatagcca
acctcatccccctggaattcctcccttgggttttcaggtaagggctctgacttctgtctg
cgtggtgttgcctgagggatgctttcttctgagtctgagcctccagaggcagccagggca
gcctcctgtctaagccttgccaaggatggataaattggtgcatagggggtagagtttcta
actcttcagatggggcctgtaggacaggttttttctgcttttcctgtgactctttttctt
tttctgatgacttgcagtctctttctattgttcctggttttgtccaagccattaaagtct
tatggtaggtctcaaagcaggtctgcagccacttagggcgggtctgaattacacttagct
gggagtctgtatacgggaactgatctgggtgcctgggctattctccaagcccagtgacca
cctgaaacacttggccaattatctccttgtgtattgtcccctcggccagccaccctacat
taaaagacagccggtttatctcacaaaaggttctcagtttctgtggagttagcttaacca
tttaatcaccatttaaaccttttgtaaaagtttttcagnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnattcctgttacagctagctgcagccatggtggatgctgcttagccaggagagtac
cttaattcctgttacagctagctgcagccatacnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacacacctccccc
gtccctgaaactgttttcctttctgactgacgtgcgagccccacctgcatctggtgtcgg
ttagggcgtgagttttgtctgaatcaatgggcctctcccattgtcccaaccccctcgggt
cagatcagtcatcatgccctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnntggttcagatggctgtgcgctgctcccgtgacc
atcctgcaaccccttccactggttccatttgtgctgtcgggggaaggccccagaacgcgg
gagggcagttctccttctgggctgaaactctcctggtggtgccaaggaccccagatctcc
catgtcctggggctgtagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
ggatgagcccccagaaatgttacaagacaatcaaagactggagagactgaaaaaggttca
ggagagtctattaaggtgatcaccggcccagccggacatatgtccagaaagtatgagccc
caaactaagggcttttcctacttttaaacattttaaggcaggaactacatgaggtgggaa
gcaagttacagaagcgagaaacaaaggcagttaatcaagcattacaacatttcttacatc
ttgagaaaaacatgtcttgcaacctaaacttattggtcttgtgactgcagctgtgcagga
gcttgctggcctgtaataaacttgagtaatttggagttggggagtatagataaggtccac
tgtccacagagacaggacaggctgttaacattctcctttaacttgagtgtaagggggcag
ggatcacactttgcagaaactttaagaggattttaaaatttctattactactactattaa
gttacgtttgatttcattaatttcttcttcaaattctaggaagtctgatcccagagttaa
tggtgaatgattacaaaacttatataatatctcacatatggttgtttatattttccaata
aagttatatggggtgaataattataacaagtatttctgaaaaaaataacaattgagaata
ctgatatatatagtccacagaaataatgagggaattttaataccatactccacattggct
gaaactttctcactgcaggctatgctgtcccattcaacattctataaactaaagatgata
gggagaatcttgtttaatctgaatgggagcttgaaggatacattaaatatggaaaatatg
catccttccttaaagagaagtagcaaatgttttcattattcatttattcaaccacttagc
caataatgttaacaattaaaggtgggagcattaaaaaaaggaaaagtggggtggggcggt
gaaatgaacctcactgatttcttgcttttatcatttcaaactccaaaagtgtatttcaac
tattcatatgatgattgaataaacttgttttacattatggaacacccttactgtgtagag
ctgtgagaaattcaataatcagtatttaatgtggacaaaaatagatagattagagaaaca
ataaacatggacatatctacacataatatatactccttttctatccaaaaatctgacaaa
aaccttacacaatggtcagtttacaccattttaaggacaaggatgtggtgaaacaatttt
aaacatttttatataataaaagaaaaaagttgttttaattgctaatgatcaagtttttac
caagaatttacatatgcatcatcaaacagtagcttgaaactgaagcttgacctttgagaa
ataaagcttttaaagtaaatgtctaaattctcttttctttattatgttacatttcattta
ctttaggttttctagtgcaatttaaataagaaataaatagagagtatggtaatcaggtac
aaaggctgtgaaaatattccctgcattttgcacagggaatatgttcaaattcctaatctg
caaagaagaaaagtgcattttcttttttgaaatggcatttgaagatctctccatccatga
acattcttgagcatatggattttacaataacagcagtgtatattagttttttaatttatc
attaactgataacatatacagtaaaatataatttccgttttcttatctaagggcagaaaa
tcccaacctatttctgaatctcactttggaacaaggacagaatgacctgacatggcattt
ttgatttccaacgtttaaacgcataacgtttgtgtgctatggaagcatcttagcttctgg
aatttatgctcgacacgaaacataaacaataaacaattcagtccacgttatatagacata
tctcatatatatatgagatatatatatataaaatatcttacgcacatgcacacttagact
ttctcggttttcatgaaactcacaatctacctcaggcgctcaaaggcactcggcctctca
ggtctgaggcaccacagagaggcttccttgggcacagttgcttgctggtcaagacgccaa
cttggcaaggttatgctgctggcaaaggcagattcgtcagaataaagtcgccacaggctc
aaccaggcaaatcatgaatggccctttcagcaggagcctgagaggagggatgttattcag
cccagcaaccctatttactttttttcaggatccccaaactcgcgttttttaaggctttcc
gctacaagagagccagaattgaagcctgggttggcggcagtgcaggtacctgctcaccta
agcatccctcttttaattttcctaactcctccacccacttgcccaagtataacttttgaa
tggattcagcagaagtgaggcaggaaaggcaggaagatgagaaaaggcgcgacatcaaca
cgcagagctcactgagccgccttgatgataaaaggcgggcacagggactacgtgggtggt
ggcagaaagggcgcgggacacgcctcgcaaagagggaagagtgggcggggccacgtgccg
ttgtcagagttcgcaactcgagcgcccagagggctcgcgaaaagtcccagcctgcaagcc
aacctcgctcagcggacgactggccggatcccaacgcgctgccccttgcccagcctgcga
gcgcgtggtacgaaggcgcgtctgcatccatgccccagcccggggagctggaggcgctcg
cagtcagaggcgagtgatgctaggctgagcgcgtggcggcccgtgtcgtgccccgctgag
ccaagtgcggaagggcagcggcgcgctccgactctgctcgccgcacgcagggcggggcgc
ggctggggggcggggggcctggccgcccgctgggagctgcggacgagcaggcgcgctgag
gacccgagggaggacacggttaaagcattgctatcaactgtgaacccagagagccctcct
tagccaacacgctaactccgaagcctcccttacgcccccgaaccaccgaaggcggcgaca
cctgattcagcgcacaaacacaggtcccttctgtcccggatacaattacgcggcagacac
acactcaaactcgcgcggggcagccaagagacgaggtgagcggagggaccacgccgttcc
agagggcggaagggggggcggtctgggtggaggagaggggcgttgtttgctccctgaggt
gagtgccgggcgaatggctgctgtccaggggcgggggggtgggaattagaaagcacagac
agggttgggctgaggaagttagaagtggttagtgggggttggcggggtagggagaaaggc
taggggttgagggggtgtgaaggagatctttttaggctggcccctcatagcgcccgcgat
cctgtttctgccatatggcatccgttcgagggttctctcgcgtttgtgcgctcaaggcag
agggagggtataacggggaggcgattcggccgaggaccttgtacttgacccctgaaggga
tgggcaccgcggagcccagaggccagagccagctgccagggcagggatggaggtggggga
ccaggaggagcttcgggacctcttcaactcccctttactccgtcttccccctaaggagga
acaagagcgggatgtgttgggggtggggaatgtcttggggctgtacaagctcgctgagac
ttttcggggcccgccgtttccgcgtgcccgcgcgactctcgacaatggacagtgtacagt
ggtggcagggtttcacgggtccccgcgccgatttgggaatggtacggggtgcccttgcat
tagagaagcctgtgcgggcccccgggattagagaagaggtgagcgcctgcgggcggggag
agaaggacgcgctgctggcaaaaagaagggcgccttccatggactggaggagcggcgagg
gagcctggtatgtgtgttttggatttgaaggaggcaaaccgcatcggagagtgcaagtag
atgcagtgcgtcggggcaaaaggacaatctgaggaggtgggcggggtacccaatctctat
atacaagctttctggtttaattgagggtttataaaattgagtacggtaaacctgccattg
tgcagcacttccttaggaaaggaatagcctctgtacctgtaccgcgagttcccatcacct
ttcagcatctaggcggggagaggagagaaggtagagaacttctgagagagttctgagaac
taacgcgaatggctggattgcgctaactaggcgttcttagcaggaaaatgtcgccaaggg
aaggagtgggtggcggtatcaaaccaggcgtgctctggagaatcacctttattatgagcc
agtgttagcagtgacgatagagtcttttatttctggggatcctattggtaaaaattagaa
aaaaaccacactactccaaatactccaaaaatcttcagtcccctcaggctgtcaccttgt
actaccagtttttcagtatcaatgacgtgaatgttagggagtttggctagatgccaaatt
gttatcatttctgataatttcatgttcaaaaacgtctatattcactcaaaattggcgtgc
ttctgacatatccccagaaaaaaatcaccaccatttgaaggaaggatagaaagtgccctt
ttttcttttcttttttaaaaacttttattttaggttttnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnaaccaatattttaatagtgttaagagtaaccata
ctttgtccccaggagattttaaaattagagtcatgcatcttaatattatatggtaaagga
gaccagaactttgtgttacttgaattaaatcttagccagcttagttttctcctatagaaa
ctgtctcttggtggtttgaggttttgctttaggaatgtgtctgtgttcagcaactcttct
agataatgccagagaaaaacactctagtattgtatgatttgcctgccacctacaaggtta
agttgacatctagtgttgtgtgacttagtattatacaagtctttaaaaactacaataaag
catagtgccccgatatttagtaaaggcttgatactctattaggaggttgggcataactga
acagaaactatacttctgactttttccaggtaatactactatacaggttcaaactctttt
taaatctaagatttccagaggactatgcttgttgtatttaagcttggtacgcgtcttaga
ataaataaaccaatgcagtaaataaagatgcttatgacaatttttttggataaaaactac
ttttcgtttaaagattttgacagtttacatttcccaataatttaaattaaaacattttat
tgcaccttatgaacaactatcagcttcattaccatttaaatattgctaaaaatcccatct
tcaatcctcaaatcctttttaaaatcatgcacctgtttatcaaataatatcaatttgttg
ctttttaattcatactaggattttccaacatacacctgggaatttctaagatatccttgg
agagtgagaactttgattatttatgcaagatatagtatctcatttatttgacctcatctg
cattggtacttatttacataagcacattttctcggtagctgactcttcacaccgtttatt
actgaaacttaaaaaaaaagagccattataacccacaagcaatttctaaatcttactgtc
ttccttgttcccttggagaatattggatataagttattctttcttgttgggttgtcttta
tgaattcataaagaattgaatagtgctattcgctgaggtgtatagagctgtttttgttta
tagtaaatggtctcagcctgctgggttgaaatactagaatagagacagaagtaccattct
tctttttatgttttttttttttttctgggtcctctttctgttattggctatcactttctg
ctgctgtcagactgtgagtgggccctgctgctctattatctgttgtctttttttccatca
aatgatgtgggctattgcccgagtgtaaatcctgtagactttgtgacagacactttcagt
ttacgtgctttatttgtggtttggactgtgaactgaaatgtttaggtttccagaagggtg
acctctatataaaaggttttaatgtaccttttatagaaaaaatttgtgtatgagatcttt
aggagggtgtattaatttcagacaattcagtcttctggaatatcttggtgtttctgagaa
actgaagcagcctttaaaaaaaagtttactttcttatgataggcccagatcgtctttgtt
taggaatatgtatactagaatattttacatattactttctgccattgaaacttactaact
ataatgtagtgtagttaatatttttctaccccggaagtccagaataagaagtatgatttg
agaatcctgctaaccatacttgaggctacatgaattatttaaatattattttgcaaatct
acttatggaggaggtacataatcaaatacatatgattaaaatggtttaatttgatcttga
aaacagtggtaatcatatctccttaatctgaagcatttcacaaacattcattcttttatt
caacaaatatttgttaaaagcatactgtgtgccaagcactgtgctctggggatgtgcata
caattaaggccctgggagggctctaggtttaaaagggggagacaaggaggaaacaggcta
ttttcaatatctacagtatagtgctggctgttctgtaagtgccgtgatagagatacacac
acagtaccctgagcagaaatttggaagtggtcaattctgtgtggggacaggagatttgag
ggtttcttagagggtgacataaaagatgaatcactggaggcaggagcaagatcatggagg
gctgtttgccattctcaggagtatggactattttcagtttaaggttgttttaaacagagg
agctatgtgatgagaaaacagctcttacagcaagcaatctagaaggatggatctgaggaa
tgtgagatttggatggtagtttagaagatattggggtggctttggtaagagaggttgagg
tccttgtaccaaagtgttggcagtgggatgaaaaaaaggggtttggttacaacgatattc
aggtggtagaacctctaagattaggtgaccaatttggttggaggattgggagtgggtggt
ataggaggatgggatgggaacttaaaaggagatccagatttgagagcaagagtgatagag
ttcagttttagacatgtagaacttgaagtgcttatagaaaatcgaactggagatgtgtgt
ccagtgaatcattgtatgtgagttctgagcacagaggtcctgggctagataggcaggtag
ataaatggtaggtagataaatggatagacacacagaaacatttaggagttttcagtgtat
aggtaattataaaccgtgaaagttgataggttttccagggcaagtaccaaaagttaatag
ggaaacagaggcctggggagatcctaaaatttaagagttgggtagagaaataggaagcaa
taaagaaaaacgagaaggtacagaacaagagaggcaagaggagagttaggagggtgtaac
ttttaaaagcagctgaaaactgagaaaatttcagaaagtgagaaggtcaggtactgaaat
gagggggtgttagacagtctcggttcccaccccctctcgagccccgaatagctctgtgac
cttgagctcacaatttagtttcatttcctcatttagcaaatgaggggagaagcacctact
ttatagggctactgtgaggatcagatgagataaaatctatcaagcacaatgcctgggagg
ttactcctttgtgtttaacaaatgctacctgttttatggaaaccaggacttggatttgtg
attagaaggtccgtagtcatttttgctttcacaagtactcgctctcagaattcacagaga
gtgattgaaaatatatttttaagatgaaaatttgatcatacttttaacttttttggttag
aggccagtagaatagcagccattgaaagttttgaagggagcagagggagccaactgaaca
aaagtccttgaagagagagtggaagttgcctcaagagcgttgcagagatgttgggtttct
caggacaatccatgcttccgccagtcaggagggtctggtggcatatgcattgtgtttaaa
aagcacctggtaattaaacctccaggtttctaatgtcttgtttaaagtactcttagtatt
ttaagtgtgttctagttccttagataatctataaggacattgattatacattttagtatt
agctgaaaataaactcagaaaacaatacagatgatttcaaaatttaacaaaatgtaattt
aatatttgatgattccaaaggcacctctatacctcatagggtcttaaaaatcaacatgtg
aaaaataattattagcctgtgtaataggggaaggactgatttgtagaggtttgtttgctt
tttttttttttttcccccaattaagactactatggtaatatttaaatatacctacttttt
tcctaggattttctgtttggatgaagtgatgctttatattagagattaataaacatttta
aattagatttctattttaatatgtgtataagttttttactctgtaattactgcataattt
gagaaaatgtttacatctatgcagtggtttggaagtcaaccaacaattgcacatagtaca
tattgtttactaattggttataaggcatataataaatatttgttgtcccaatacatgttt
gttgaataaaacaaaattagatattccatatttttaagatgaaatgaattttatatctgt
agttttaatatttaatcatattctttacctgaaaatcgctagccatacttttcgttttca
gcacttctctattttgcagttgtgtttcagtgcttttttttttcccactgtgcttagtcc
ttcacgatttatggccctgttatttgtaaagcatagtaatctaagcaggtgattttcagt
ttaggatttcttctagttccatgttgtatattcaccaagtcagttttagactatatattg
tcatgctcagcactcccttacagagataacagatgccaagttgaaattgacttatacaga
atgccacacttaggtaagtgtttaggagtgcatagccaaaataaaaatggtacctttggt
ttatgaatggcagggcatctaggacagtgtaggtcagttcgtattctctggatggacatt
tatgtaaatattaaaacatttagattagatggaaagatgtatagtaggaacatttcagag
atactgtttccttgtagcattgtactatttatctgaattgttaatttggaataatcacta
tattgattttattttgaagatataatttatttccattgacttgatataaataacagaaat
gcatctgcaaggaatccagttcattaaagaaatttacattcgtatctgatttggtaaata
tgtgcttgatttgggaatattatataacaaaatatttgtgaaatcattttgtacttttta
attgttttgaagaaatttaatactcaaaatatttcaagacaccaaatcatgactttttag
ttattacattaagctataacttgctttaatgagtaaaaactgtggggctaattttcttcc
aagtgaaacatcaagtttaaataatgtgcacatgaatttacaaagtagaatcaagtgtgc
aaatatgctatatgttctgtaataggttcttgaaaagctgtatataaactcagcttttat
aagttgagtcatatttatagaataagaaaatagggcaaacatttaacacttcttaaatat
taagtgatgggcatacagatgtttgttatgttaatctttatatttttctgtatgttagaa
atagtttacagaaaaaaattgtggttagtgacattaaagcacattatatattaaatatac
tcagggactttgaaatttaaagcaatatcaacatacaatttaccatagaaaaaatcctat
tctgattttattaatacctgttttgcaaattcagaatttttaatgggtttaattaagcag
atacacacacaacaacaacaacaacaaaaaacttggacacacttctgtaacacatcttga
atatagtctcatctgttggaattttttaaacttctacacttaagtcttaatctgattctc
cctcatcctcataatattcattattcagaggagaggcatcaacaggaagaattcaatttt
acttaaataaaaattttttactagcaacggaatgagtgattcagtggtggtgtcttcctg
ctttgagagagctgttctaagactaggcagttagtcttaggcctaagactaggcagttag
tctaggggctccacagcaggcccttcctctgggttctctttatgaagaaggcctttgtgt
tttacatagattttgcatttgtgctgctgtccctattgagttgttctcaccaagccaatc
tggggagtagctcttaggggaaaactagtcaaacaggtttgcctgtctattcaaaaggag
ggaagaacggtgaaatgtatggataaaggccaagagtaaaactgttttaagtaatattca
tgctaatcactgactgttggttttgaaaatactttgtagacatttgtgagaatgtatctt
aatggagataaagtaagagagaaactggaaggggaagtagtttagaagcaggggataagg
acaagatggggagaaggaagggaaaaacaatggaggcaaaataagctcaagaaggaaaga
atagtgtttcagtgtcatatctgggctttatgtaaactgggtgtgttatagcaggaaacc
atggtattgatctgagaatgaatttaattaaaacaaacaaaaaacccccacatgcctgtg
atcctcaatgtccaaaatagggagcgtcttgccagggcagggctgaagtttactaggaat
attggtgggaaaatgttatagttaaccatctctgatactaatcttatattagagataata
cttggtttcccctagtaaagtgttagagtcatacagctttattgtagtttgcaaatctat
tacacatatatttctactgtttagttctcattctgcccctgaagcatagccctataggta
gtagcattttacagctcataacttggtagagtcccaagtaacctttggaagtcaaaatga
caatccagtctggggaatttgatgttaaaactaaagcttcttgcctagaacaagttttgg
aactatagctcaccacctgcctcatgttctgggaatgctgaaccttgatcccactgaaat
tacctttaagtagtctttctgtagtcctcagtaatctcctcctttgctccatgcttcttc
cctgctccttgagagttccttctcctttctcatctgtaatggtaactccccatctgatct
ttggttgtgcctcttcttccttcctggcatttttattaacatcttttgtcccagcacctc
tcctcttctctattctcactcctttggtgtagttattcatgcccatagactggattttta
tttatatatagatgattcaaaaagctgtatttctaattgtgatttcttctatactctcaa
gagcttagagttcccagctcaacttcctaactcagatctgacatccccatttgcctcagt
cttcgaagacagattttggtgttgtggaattcacttttttaccctttatatctaatctat
tcctaattgatcactatgtccttcatgaagaccttcttcccttgcctgagtctttccatt
cacctcctgatgtcttggtgtagtcccacatcacttcttcaccagtggtactaaatgact
cattttcaccattacctgggccttccatcacctcctactgcccacaaccatcagtcactt
gtccttactgacccacttgccttctttgagtgtgatttgactatgcgtttattttcacat
ggggatccaaaatttttagcatggctttcgaggctgtctattgtctagctgctttctgat
cagacttatttattttctcatattgctcaataggattctatcctgtttacccactggggc
attcatttcaggatatccccccgcttgagatgtcctgccttttttttcttctgcacttcc
aaattctcacccaaggttttgacactcaagtcccacctcagactgtggcttcccagtctc
tgtcacttttctgtgactttgatttgtacatatattgatgttagcaattaggtttcattc
tccctctaaggagagtatgaactcttagagggtggagtctcttgtctcctcatacacctt
ggacaaacactattgatcagagagaagatattcaaacatgattgaatggtatctttagtt
tgtcatgaggcaataagggtaagtttcctaattttctatacgtattttgattttttcctg
ttgttttttccagaactgcatcattcaaacttgaaacattcccctatttttttttaacaa
ggtgttctatataggaacaacatatcttatagggattgctacattatttagtcacaatgg
aaaaaaaagtttgatatatagaaagattatataatagttacatggaactctactataaaa
ttataatggtttagtgggttttcttttttaaatttacctggggcttctataggtggatgt
gcattcacaactaattctaggtaatgctaataaaagaaaataatcacggtgataattaac
tgtatttagtttttattttgtatttacatttcaatgtaagtatgcctaatgtcatgaaac
catagttggagaaaataatgattatatgcatgaggaagataacttaggaagcattctaat
tgtccaactccccacttccccatagaccttttataacaattaaatcaaagcaccagccca
ggaataattcttttttccattctttgtgcctatttcctcctttgataattgctgaattct
tctggtcagaatatctccctttccatctccctctgagcagagcaataggaataggaatat
attttaagccaggtgaagtggtgcaggtctgaggtnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaaaaa
gaatatattttaagaaaattcctcagctaaagtaaaaagaaaagagagataagctaggta
agattattaatcagaagtaatgcttgtctgtttaatttgccagttttaaataattcttga
aggcatttgagaggtcattatattcttttaatcagttagactttatctgaatatatgctt
tggaatatactttttcttcaaacttctaaactatgtgcaatatacagaaacaaggtcctc
tggctgtcctgctttctgcttgctttttttctctccctttcacttttctggagataattt
ttctgcctcttgtagggaattcagaggagggatcctagtgattggctttacaaatttgtg
tgggggagggattggatagagtggagatgggataggcatctgcccatcagtaggatttgg
ttgtttgtccagcctctatatgggctttaaatgctttatactttctgaccattatttttg
ccagcaaaaatgatgtagcttattttgatactacttaaatgcagtcctgtccttattcta
cccctactactccatccaaagggagactgaaggggagacattttgaaccagacatgttga
tttcctggtggcctgtacagtgagtattttgtaacatttagaattaattcagtcattcat
ttaataagtaacctaatatatgctagaaatcttctgtcttagagccaaaatatattgtaa
ttttttcatggtctaagtatctaaggctttacagtataaaagtttaataaagtcatgacc
aaggctttacaaagactgctattttgattcatgtatcacagaattcaataaaaagtcaaa
ctattatctgacatatggcagtgacaactttggagggtgtttatatggtacaaatagaag
atgctggattaggacctaggaaacctgaatgctggttccgactctgccagcgaataactg
aactagctgtgtaatgcaaagacaaccaccccatagagactttgcccctttctctacaaa
tgaggggtttgaaatcacctatctttagtttttaaattgtgactcatttatttaatgaaa
atatatggtttttaaaattttgatatcattttggtacaaatatattgtgtcaatgtttaa
atatatttatcttattttcacaagacaatggcaatgatatacaaaagactttctctggac
ttgcctcattgtggtgaatatcggttataatcatagatacttagaagacacaaaattata
cattcttacctgttagtaaaggaacatcaaagaaattcattctgtttttctgcagaaact
atcaaaatttttccacttctggggattaagataacttctgttttttatctaatactcaac
ctatctaatacaaccttgtcctagtgatgatggtagaattttataaaatagggaagtttg
ttatttgttcacctgtgaagaccttcaaggaattgaataggaaaatacctagagttttgt
ttcttgttctgatttgcttgtttatatttggccagttttgtcctgaaaatatgtttgaga
gattacatgaaaatttattttgtgactcttaatataaatgttaaaaatacatttaaacta
gtgatagtaaagaaagaatatcttaattagtgggggaaataatcccatgatcttttgtga
ttctacaacattactgtagccttaagaatctctactaatgttgtattgaaaagctgtttc
tcctacatgtgaa
--------------------------------------------------------------------------------
# Output_end: pretty

gc_object_end: repeat_grailexp --output pretty




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