[Bioperl-l] Ask help for Bio::Tools::Run::Primer3

Fengkai Zhang (Tony) fzhang at vcu.edu
Fri Oct 1 10:54:49 EDT 2004


Thanks for your reply. It still has problem after changing the file
name. I figured it out and you may see my reply.

Tony
-------------------
> Yes, it does matter... simply change the name of primer3_core to
primer3, put it in your path, and you should be good to go!
> 
> Adam
> 
> -----Original Message-----
> From: bioperl-l-bounces at portal.open-bio.org on behalf of Fengkai
Zhang (Tony)
> Sent: Wed 9/29/2004 4:21 PM
> To: bioperl-l at bioperl.org
> Subject: [Bioperl-l] Ask help for Bio::Tools::Run::Primer3
>  
> Hi, I am a newbie for bioperl and met the below question.
> 
> I was trying to use Bio::Tools::Run::Primer3. When I run the example
> code in the documentation of this module, I got the following error
> message:
> --------------------------------
> <shell> $ perl -I ../primer3_1.0.0/src/ -w primer3run_test.pl 
> 
> Use of uninitialized value in concatenation (.) or string at
> /usr/lib/perl5/site_perl/5.8.0/Bio/Tools/Run/Primer3.pm line 212,
> <GEN0> line 1.
> primer3 can not be found. Is it installed?
> --------------------------------
> 
> I already installed Primer3 according its instruction and it works
> well to run the test and example. I also noticed that the
> primer_test.pl in primer3 package calls "primer3_core" rather than
> "primer3". Does it matter for Bio::Tools::Run::Primer3 module?
> 
> 
> Thanks ahead for your help.
> 
> 
> 
> Tony
> 
> VIPBG at VCU
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> 



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