[Bioperl-l] Help me to deal with "MSG: blastall call crashed: -1 blastall ..."

[hcuong]

Hi, Please help me. I try to make BLAST search for the first time by the test script:
> #----------------
>
> #!/usr/bin/perl
>
>  use strict;
>
> use Bio::Tools::Run::StandAloneBlast;
>
>  my @params = (program => 'blastn',
>
>                             database => 'ecoli1.nt');
>
> my $factory = Bio::Tools::Run::StandAloneBlast->new(@params);
>
> my $input = Bio::Seq->new(-id => "test query",
>
>                                              -seq => "ACTAAGTGGGGG");
>
> my $blast_report = $factory->blastall ($input);
>
>  print "$blast_report\n";
>
> #----------------

But I always get the following error only:

>  ------------- EXCEPTION  -------------
>
> MSG: blastall call crashed: -1 blastall -p  blastn  -d  /ecoli1.nt  -i  /tmp/bSh3ZAfK7n  -o  /tmp/gv9wpcMj8g
>
>  STACK Bio::Tools::Run::StandAloneBlast::_runblast /usr/local/ActivePerl-5.6/lib/site_perl/5.6.1/Bio/Tools/Run/StandAloneBlast.pm:633
>
> STACK Bio::Tools::Run::StandAloneBlast::_generic_local_blast /usr/local/ActivePerl-5.6/lib/site_perl/5.6.1/Bio/Tools/Run/StandAloneBlast.pm:602
>
> STACK Bio::Tools::Run::StandAloneBlast::blastall /usr/local/ActivePerl-5.6/lib/site_perl/5.6.1/Bio/Tools/Run/StandAloneBlast.pm:489
>
> TACK toplevel blast_local.pl:15


---
Nguyen Huu Cuong
Plant Cell Biotechnology Department
Institute of Biotechnology, NCST
18 Hoang Quoc Viet, Cau Giay, Ha Noi
Tel: 84 - 4 - 7562368
Email: hcuong at ibt.ac.vn