[Bioperl-l] e-value from RemoteBlast differs from hand made b last

Martin Schenker Martin.Schenker@ogs.co.uk
Wed, 22 May 2002 12:28:00 +0100


Might be a stupid question, but have you checked that you are using the same
BLAST version and database in both instances?

The BLAST version is given in the first line of the output like:

TBLASTN 2.2.1 [Apr-13-2001]

The database stats are given below the Query:

Database: /scratch/db/my_strange_db
           719,859 sequences; 375,894,912 total letters

Best, Martin

> -----Original Message-----
> From:	Carsten Daub [SMTP:daub@mpimp-golm.mpg.de]
> Sent:	Wednesday, May 22, 2002 11:57 AM
> To:	Perl - Bioperl
> Cc:	daub@mpimp-golm.mpg.de
> Subject:	[Bioperl-l] e-value from RemoteBlast differs from hand made
> blast
> 
> Hi,
> 
> I blasted with RemoteBlast and the score/e-values I obtain differ from
> the ones
> I get by a hand made blast at NCBI.
> Is there a way to get the the same score/e-value by RemoteBlast as by
> hand-made blast?
> 
> Carsten


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