[Bioperl-l] Error from Blast

Andy Nunberg anunberg@oriongenomics.com
Mon, 30 Dec 2002 10:19:41 -0600

Every so often when I am running Blast via Bioperl i get this sort of error

[blastall] ERROR: ncbiapi [000.000]  sequence.ab1: SeqPortNew: gi|16578911
start (-13194311)< 0

I usually get a few of these on one of my sequences and then BLAST crashes
and I see the exception thrown up from bioperl.

Anyone know what this means? The sequence itself usually doesnt look so
bad. I know that if I re-run the blast without word filtering, this usually
helps, but not always

Andy Nunberg, Ph.D
Computational Biologist
Orion Genomics, LLC 
(314) 615-6989