[Biojava-l] RE: Sequence serialization exception - AlphabetManagerproblem?

Schreiber, Mark mark.schreiber at agresearch.co.nz
Tue Sep 30 06:27:40 EDT 2003


Hi -
 
I was a bit thrown off at first cause I thought you meant there was an error in binary serialization. There seems to be a problem with SwissProt writing. I've commited an addition to SeqIOToolsTest in biojava live that replicates the error but I haven't got time to track it down just yet. If some one else doesn't get it I'll probably find it tommorrow.
 
- Mark
 

	-----Original Message----- 
	From: Mark Southern [mailto:msouthern at exsar.com] 
	Sent: Tue 30/09/2003 10:45 a.m. 
	To: biojava-l at biojava.org 
	Cc: 
	Subject: [Biojava-l] RE: Sequence serialization exception - AlphabetManagerproblem?
	
	

	Appologies for following up on my own post. What follows is a simpler test
	than the serialization I attempted before.
	
	Consider the bit of code below and corresponding error message;
	
	For some reason, the protein sequence is being treated as a dna sequence. Is
	there something I am missing with respect to how AlphabetManager treats dna
	and protein alphabets?
	
	Any explainations would be most welcome.
	
	Thanks again,
	
	Mark.
	
	
	//------------------------------------------------------------------------
	
	public static void main(String[] args) throws Exception{
	        String seqFile = "c:\\temp\\KAP0_BOVIN.swiss";
	         SequenceIterator iter = (SequenceIterator)
	SeqIOTools.fileToBiojava(SeqIOConstants.SWISSPROT
	
	,new BufferedReader( new FileReader( seqFile ) ) );
	        Sequence seq = iter.nextSequence();
	        SeqIOTools.biojavaToFile( SeqIOConstants.SWISSPROT,  System.out,
	seq );
	}
	
	
	org.biojava.bio.symbol.IllegalSymbolException: Symbol ALA not found in
	alphabet DNA
	        at
	org.biojava.bio.symbol.AbstractAlphabet.validate(AbstractAlphabet.java:278)
	        at
	org.biojava.bio.symbol.AlphabetManager$ImmutableWellKnownAlphabetWrapper.val
	idate(AlphabetManager.java:1423)
	        at
	org.biojava.bio.seq.io.CharacterTokenization._tokenizeSymbol(CharacterTokeni
	zation.java:178)
	        at
	org.biojava.bio.seq.io.CharacterTokenization.tokenizeSymbol(CharacterTokeniz
	ation.java:191)
	        at
	org.biojava.bio.symbol.AlphabetManager$WellKnownTokenizationWrapper.tokenize
	Symbol(AlphabetManager.java:1276)
	        at
	org.biojava.bio.seq.io.AbstractGenEmblFileFormer.formatTokenBlock(AbstractGe
	nEmblFileFormer.java:337)
	        at
	org.biojava.bio.seq.io.EmblFileFormer.addSymbols(EmblFileFormer.java:211)
	rethrown as org.biojava.bio.symbol.IllegalAlphabetException: DNA not
	tokenizing
	        at
	org.biojava.bio.seq.io.EmblFileFormer.addSymbols(EmblFileFormer.java:224)
	        at
	org.biojava.bio.seq.io.SeqIOEventEmitter.getSeqIOEvents(SeqIOEventEmitter.ja
	va:125)
	rethrown as org.biojava.bio.BioError: An internal error occurred processing
	symbols
	        at
	org.biojava.bio.seq.io.SeqIOEventEmitter.getSeqIOEvents(SeqIOEventEmitter.ja
	va:137)
	        at
	org.biojava.bio.seq.io.EmblLikeFormat.writeSequence(EmblLikeFormat.java:289)
	        at
	org.biojava.bio.seq.io.EmblLikeFormat.writeSequence(EmblLikeFormat.java:253)
	        at
	org.biojava.bio.seq.io.SeqIOTools.writeSwissprot(SeqIOTools.java:316)
	        at org.biojava.bio.seq.io.SeqIOTools.seqToFile(SeqIOTools.java:1078)
	        at
	org.biojava.bio.seq.io.SeqIOTools.biojavaToFile(SeqIOTools.java:870)
	        at com.exsar.test.SerializeTest.main(SerializeTest.java:24)
	
	
	
	-----Original Message-----
	From: Mark Southern [mailto:msouthern at exsar.com]
	Sent: Monday, September 29, 2003 2:01 PM
	Cc: 'biojava-l at biojava.org'
	Subject: Sequence serialization exception
	
	
	I am getting the following exception when trying to serialize a protein
	sequence. I am using biojava 1.3. Can anyone please explain to me why?
	
	Many thanks,
	
	Mark.
	
	
	java.io.InvalidObjectException: Couldn't resolve symbol:SER
	        at
	org.biojava.bio.symbol.AlphabetManager$WellKnownAtomicSymbol$OPH.readResolve
	(AlphabetManager.java:1441)
	        at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
	        at
	sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:39
	)
	        at
	sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl
	.java:25)
	        at java.lang.reflect.Method.invoke(Method.java:324)
	        at
	java.io.ObjectStreamClass.invokeReadResolve(ObjectStreamClass.java:911)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1655)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at java.io.ObjectInputStream.readArray(ObjectInputStream.java:1603)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1271)
	        at
	java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1845)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1769)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at
	java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1845)
	        at
	java.io.ObjectInputStream.defaultReadObject(ObjectInputStream.java:452)
	        at
	org.biojava.bio.seq.impl.SimpleSequence.readObject(SimpleSequence.java:119)
	        at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
	        at
	sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:39
	)
	        at
	sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl
	.java:25)
	        at java.lang.reflect.Method.invoke(Method.java:324)
	        at
	java.io.ObjectStreamClass.invokeReadObject(ObjectStreamClass.java:824)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1746)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at
	java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1845)
	        at
	java.io.ObjectInputStream.defaultReadObject(ObjectInputStream.java:452)
	        at org.biojava.bio.seq.ViewSequence.readObject(ViewSequence.java:93)
	        at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
	        at
	sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:39
	)
	        at
	sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl
	.java:25)
	        at java.lang.reflect.Method.invoke(Method.java:324)
	        at
	java.io.ObjectStreamClass.invokeReadObject(ObjectStreamClass.java:824)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1746)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at
	java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1845)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1769)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at
	java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1845)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1769)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at java.io.ObjectInputStream.readObject(ObjectInputStream.java:324)
	        at java.util.HashMap.readObject(HashMap.java:985)
	        at sun.reflect.GeneratedMethodAccessor6.invoke(Unknown Source)
	        at
	sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl
	.java:25)
	        at java.lang.reflect.Method.invoke(Method.java:324)
	        at
	java.io.ObjectStreamClass.invokeReadObject(ObjectStreamClass.java:824)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1746)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at
	java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1845)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1769)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at java.io.ObjectInputStream.readObject(ObjectInputStream.java:324)
	        at java.util.HashMap.readObject(HashMap.java:986)
	        at sun.reflect.GeneratedMethodAccessor6.invoke(Unknown Source)
	        at
	sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl
	.java:25)
	        at java.lang.reflect.Method.invoke(Method.java:324)
	        at
	java.io.ObjectStreamClass.invokeReadObject(ObjectStreamClass.java:824)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1746)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at
	java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1845)
	        at
	java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1769)
	        at
	java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1646)
	        at
	java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1274)
	        at java.io.ObjectInputStream.readObject(ObjectInputStream.java:324)
	        at com.exsar.hdex.model.calc.Test.main(Test.java:104)
	
	
	
	
	
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